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Quantum Chemical Modeling of the Dehalogenation Reaction of Haloalcohol Dehalogenase.卤代醇脱卤酶脱卤反应的量子化学建模
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Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products.在大肠杆菌中操纵呼吸水平以有氧形成还原化学产物。
Metab Eng. 2011 Nov;13(6):704-12. doi: 10.1016/j.ymben.2011.09.006. Epub 2011 Oct 6.
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2-haloacrylate hydratase, a new class of flavoenzyme that catalyzes the addition of water to the substrate for dehalogenation.2-卤代丙烯酸水解酶，一种新型黄素酶，可催化底物加水进行脱卤反应。
Appl Environ Microbiol. 2010 Sep;76(18):6032-7. doi: 10.1128/AEM.00334-10. Epub 2010 Jul 23.
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The pyrroloquinoline quinone biosynthesis pathway revisited: a structural approach.重新审视吡咯喹啉醌生物合成途径：一种结构方法。
BMC Biochem. 2008 Mar 27;9:8. doi: 10.1186/1471-2091-9-8.
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Biocatalysis by dehalogenating enzymes.脱卤酶的生物催化作用。
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Preparative expression of secreted proteins in bacteria: status report and future prospects.细菌中分泌蛋白的制备性表达：现状报告与未来展望。
Curr Opin Biotechnol. 2005 Oct;16(5):538-45. doi: 10.1016/j.copbio.2005.07.008.
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Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase ADH-IIG from Pseudomonas putida HK5.恶臭假单胞菌HK5中喹啉血红蛋白醇脱氢酶ADH-IIG的分子克隆与结构分析
J Mol Biol. 2005 Sep 9;352(1):91-104. doi: 10.1016/j.jmb.2005.06.078.
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Biochemical and molecular characterisation of the 2,3-dichloro-1-propanol dehalogenase and stereospecific haloalkanoic dehalogenases from a versatile Agrobacterium sp.来自一株多功能土壤杆菌的2,3-二氯-1-丙醇脱卤酶和立体特异性卤代烷酸脱卤酶的生化及分子特性分析
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2-Haloacrylate reductase, a novel enzyme of the medium chain dehydrogenase/reductase superfamily that catalyzes the reduction of a carbon-carbon double bond of unsaturated organohalogen compounds.2-卤丙烯酸还原酶，一种中链脱氢酶/还原酶超家族的新型酶，可催化不饱和有机卤化合物碳-碳双键的还原反应。
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PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis.PSORTb v.2.0：细菌蛋白质亚细胞定位的扩展预测及比较蛋白质组分析获得的见解
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在恶臭假单胞菌 MC4 中利用 2,3-二氯-1-丙醇的新型脱卤酶机制。

Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4.

机构信息

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands.

出版信息

Appl Environ Microbiol. 2012 Sep;78(17):6128-36. doi: 10.1128/AEM.00760-12. Epub 2012 Jun 29.

DOI:10.1128/AEM.00760-12
PMID:22752160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3416625/
Abstract

A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa monomeric protein (DppA) that was isolated from cell lysate. The dppA gene was cloned from a cosmid library and appeared to encode a protein equipped with a signal peptide and that possessed high similarity to quinohemoprotein alcohol dehydrogenases (ADHs), particularly ADH IIB and ADH IIG from Pseudomonas putida HK. This novel dehalogenating dehydrogenase has a broad substrate range, encompassing a number of nonhalogenated alcohols and haloalcohols. With DCP, DppA exhibited a k(cat) of 17 s(-1). (1)H nuclear magnetic resonance experiments indicated that DCP oxidation by DppA in the presence of 2,6-dichlorophenolindophenol (DCPIP) and potassium ferricyanide [K(3)Fe(CN)(6)] yielded 2-chloroacrolein, which was oxidized to 2-chloroacrylic acid.

摘要

从污染土壤中分离到一株能够利用 2,3-二氯-1-丙醇 (DCP) 和几种脂肪族卤代酸和卤代醇作为唯一碳源和能源进行生长的恶臭假单胞菌 (MC4) 菌株。研究发现，DCP 的降解首先是由细胞裂解物中分离出的 72kDa 单体蛋白 (DppA) 催化的氧化和同时脱卤化作用开始的。从 cosmid 文库中克隆了 dppA 基因，该基因似乎编码一种带有信号肽的蛋白质，与假单胞菌 HK 的醌-血蛋白醇脱氢酶 (ADH)，特别是 ADH IIB 和 ADH IIG 具有高度相似性。这种新型脱卤脱氢酶具有广泛的底物范围，包括许多非卤代醇和卤代醇。对于 DCP，DppA 的 k(cat)为 17 s(-1)。(1)H 核磁共振实验表明，DppA 在 2,6-二氯苯酚靛酚 (DCPIP) 和铁氰化钾 [K(3)Fe(CN)(6)] 的存在下氧化 DCP 生成 2-氯丙烯醛，后者被氧化为 2-氯丙烯酸。