University of Rochester Eastman Institute for Oral Health, 625 Elmwood Avenue, Rochester, NY 14620, USA.
Clin Oral Investig. 2013 Apr;17(3):775-83. doi: 10.1007/s00784-012-0772-7. Epub 2012 Jun 30.
To study the effectiveness of a dentifrice containing polyvinylmethyl ether-maleic acid (PVM/MA) copolymer in occluding dentin tubules and investigate the interaction between PVM/MA and type I collagen using surface plasmon resonance (SPR).
Fifteen volunteers brushed dentin discs in situ using dentifrices with and without PVM/MA copolymer in a cross-over design. Dentin tubule occlusion was evaluated after brushing, after overnight saliva challenge in vivo for 12 h and after drinking 250 ml of orange juice. Dentin tubule occlusion and tubule size were compared between the two groups using repeated ANOVA and before and after erosive challenges using paired t tests. SPR using type I collagen as ligand and PVM/MA as analyte was performed to evaluate the binding of the two macromolecules.
A median of 91% of dentin tubules were occluded after a single brushing in the PVM/MA group, as compared to 9% in the controls. After overnight saliva challenge and 10 min of erosion by orange juice, a median of 73% of the dentin tubules remained fully occluded in the PVM/MA group as compared to zero in the controls. Dentin tubule size increased after orange juice erosion in the controls but not in the PVM/MA group. SPR study showed that PVM/MA bound readily to collagen molecules in a 4 to 1 ratio.
Dentifrice containing PVM/MA could effectively occlude dentin tubules and prevent dentin erosion. PVM/MA may improve adhesive retention of intra-tubular dentifrice plugs through binding to dentin surface collagen.
Brushing with dentifrice containing adhesive polymers has preventive effect against dentin erosion and dentin sensitivity.
研究含有聚乙烯基甲醚-马来酸共聚物（PVM/MA）的牙膏在堵塞牙本质小管方面的有效性，并利用表面等离子体共振（SPR）研究 PVM/MA 与 I 型胶原蛋白之间的相互作用。
15 名志愿者以交叉设计的方式使用含有和不含有 PVM/MA 共聚物的牙膏在体内原位刷牙牙本质圆盘。刷牙后、在体内过夜唾液挑战 12 小时后以及饮用 250 毫升橙汁后，评估牙本质小管的闭塞情况。使用重复方差分析比较两组之间的牙本质小管闭塞和小管尺寸，并使用配对 t 检验比较侵蚀性挑战前后的情况。使用 I 型胶原蛋白作为配体和 PVM/MA 作为分析物的 SPR 用于评估两种大分子的结合。
PVM/MA 组单次刷牙后，有中位数 91%的牙本质小管被闭塞，而对照组则为 9%。在过夜唾液挑战和橙汁侵蚀 10 分钟后，PVM/MA 组中有中位数 73%的牙本质小管仍然完全闭塞，而对照组则为零。橙汁侵蚀后，对照组的牙本质小管尺寸增加，但 PVM/MA 组则没有。SPR 研究表明，PVM/MA 以 4:1 的比例与胶原蛋白分子结合。
含有 PVM/MA 的牙膏可以有效地闭塞牙本质小管并防止牙本质侵蚀。PVM/MA 可能通过与牙本质表面胶原蛋白结合，改善管内牙膏塞的粘固保持力。
使用含有粘性聚合物的牙膏刷牙具有预防牙本质侵蚀和牙本质敏感的作用。
