Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford, UK.
J Cardiovasc Transl Res. 2012 Oct;5(5):678-87. doi: 10.1007/s12265-012-9389-0. Epub 2012 Jul 3.
To investigate the effects of age and disease on endogenous cardiac progenitor cells, we obtained right atrial and left ventricular epicardial biopsies from patients (n = 22) with chronic ischaemic heart disease and measured doubling time and surface marker expression in explant- and cardiosphere-derived cells (EDCs, CDCs). EDCs could be expanded from all atrial biopsy samples, but sufficient cells for cardiosphere culture were obtained from only 8 of 22 ventricular biopsies. EDCs from both atrium and ventricle contained a higher proportion of c-kit+ cells than CDCs, which contained few such cells. There was wide variation in expression of CD90 (atrial CDCs 5-92 % CD90+; ventricular CDCs 11-89 % CD90+), with atrial CDCs cultured from diabetic patients (n = 4) containing 1.6-fold more CD90+ cells than those from non-diabetic patients (n = 18). No effect of age or other co-morbidities was detected. Thus, CDCs from atrial biopsies may vary in their therapeutic potential.
为了研究年龄和疾病对内源性心脏祖细胞的影响，我们从患有慢性缺血性心脏病的患者（n=22）中获得右心房和左心室心外膜活检，并测量了原代培养细胞和心脏球衍生细胞（EDCs、CDCs）的倍增时间和表面标志物表达。可以从所有心房活检样本中扩增 EDCs，但仅从 22 个心室活检样本中的 8 个获得了足够用于心脏球培养的细胞。心房和心室的 EDCs 中 c-kit+细胞的比例均高于 CDC，而 CDC 中几乎没有此类细胞。CD90 的表达存在广泛的差异（心房 CDC 为 5-92% CD90+；心室 CDC 为 11-89% CD90+），来自糖尿病患者（n=4）的心房 CDC 中 CD90+细胞比非糖尿病患者（n=18）多 1.6 倍。未检测到年龄或其他合并症的影响。因此，来自心房活检的 CDC 在治疗潜力上可能存在差异。
