Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic.
J Microbiol. 2012 Jun;50(3):401-8. doi: 10.1007/s12275-012-1621-8. Epub 2012 Jun 30.
Francisella tularensis is the causative agent of tularemia. It is an intracellular pathogen with the ability to survive within phagosomes and induce pyroptotic cell death. In this study, we attempted to prove whether oxidative imbalance plays a significant role in tularemia pathogenesis. In our experimental model, we subcutaneously infected female BALB/c mice (dose 10(5) CFU of F. tularensis LVS). Liver, spleen, and blood were collected from mice at regular intervals from days 1-15 after infection. The bacterial burden was assessed by a cultivation test. The burden was unchanging from the 2(nd) to 6(th) day after infection. The bacterial burden corresponded to the plasmatic level of IFN-γ, IL-6, and liver malondialdehyde. After the phase of acute bacteraemia and the innate immunity reaction, the levels of reduced glutathione and total low molecular weight antioxidants decreased significantly and the activity of caspase-3 increased in the liver. The level of reduced glutathione decreased to 25% of the original level, and the total level of low molecular weight antioxidants was less than 50% of the initial amount. The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen.
土拉弗朗西斯菌是兔热病的病原体。它是一种能够在吞噬体中存活并诱导细胞发生细胞焦亡的细胞内病原体。在本研究中，我们试图证明氧化失衡是否在兔热病发病机制中起重要作用。在我们的实验模型中，我们通过皮下感染雌性 BALB/c 小鼠（10(5)CFU 的弗氏柠檬酸杆菌 LVS 剂量）。从感染后第 1 天到第 15 天，定期从感染的小鼠中采集肝脏、脾脏和血液。通过培养试验评估细菌负荷。感染后第 2 天至第 6 天，细菌负荷保持不变。细菌负荷与 IFN-γ、IL-6 和肝丙二醛的血浆水平相对应。在急性菌血症和先天免疫反应阶段之后，肝脏中的谷胱甘肽还原酶水平和总低分子量抗氧化剂活性显著下降，caspase-3 的活性增加。谷胱甘肽还原酶的水平下降到原始水平的 25%，而低分子量抗氧化剂的总水平低于初始量的 50%。兔热病诱导的病理变化对肝脏的影响比脾脏更为广泛。
