增升平/ACAPHA 对恶性脑肿瘤生长和 Notch 信号通路的影响。
Effects of Zeng Sheng Ping/ACAPHA on malignant brain tumor growth and Notch signaling.
机构信息
Department of Pathology, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Building 558, Baltimore, MD 21205, USA.
出版信息
Anticancer Res. 2012 Jul;32(7):2689-96.
BACKGROUND/AIM: Zeng Sheng Ping (ZSP) is a traditional herbal remedy used to prevent progression and growth of neoplastic lesions. It has been shown to inhibit Notch2 expression in a murine lung cancer model, leading us to investigate its therapeutic potential in Notch-dependent brain tumors.
MATERIALS AND METHODS
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), apoptosis, and quantitative real-time polymerase chain reaction (RT-PCR) analyses were performed in glioma and medulloblastoma cell lines, and morphological analyses in DAOY flank xenografts.
RESULTS
ZSP inhibited brain tumor growth in vitro, in part, by apoptotic induction. Down-regulation of the Notch2 receptor, the pathway target Hairy/Enhancer of Split homolog 1 (Hes1), and of the stem cell markers Nestin and CD133 was also observed. Reductions in tumor mass and increases in the necrotic fraction of DAOY xenografts, in mice treated with oral ZSP were also observed, but these were not significant.
CONCLUSION
ZSP can block brain tumor growth and the expression of Notch pathway members and stem cell markers in vitro.
背景/目的：曾升平（ZSP）是一种传统的草药疗法，用于预防肿瘤病变的进展和生长。它已被证明可抑制小鼠肺癌模型中的 Notch2 表达，这促使我们研究其在 Notch 依赖性脑肿瘤中的治疗潜力。
材料和方法
在神经胶质瘤和髓母细胞瘤细胞系中进行 3-(4,5-二甲基噻唑-2-基）-5-(3-羧甲氧基苯基）-2-(4-磺苯基）-2H-四唑盐（MTS）、细胞凋亡和实时定量聚合酶链反应（RT-PCR）分析，并在 DAOY 侧位异种移植中进行形态分析。
结果
ZSP 通过诱导细胞凋亡部分抑制脑肿瘤的体外生长。还观察到 Notch2 受体、途径靶标 Hairy/Enhancer of Split homolog 1（Hes1）以及干细胞标志物 Nestin 和 CD133 的下调。在接受口服 ZSP 治疗的小鼠的 DAOY 异种移植物中，还观察到肿瘤质量减少和坏死分数增加，但这些变化并不显著。
结论
ZSP 可阻断体外脑肿瘤生长和 Notch 途径成员及干细胞标志物的表达。
Zotero 插件