Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164, USA.
J Immunol. 2012 Aug 1;189(3):1340-8. doi: 10.4049/jimmunol.1200442. Epub 2012 Jun 29.
Accumulating research indicates that B cells are involved in anti-tumor immunity. Chronic alcohol consumption is associated with decreased survival of cancer patients. The effect of alcohol consumption on B cells in tumor-bearing hosts is unknown. Results in melanoma-bearing mice showed that chronic alcohol consumption did not alter the percentage and number of B cells in bone marrow, spleen, and lymph nodes but dramatically decreased B cells in the peripheral blood. Alcohol consumption did not alter the development of B cells in the bone marrow and did not affect follicular B cells in the spleen; however, it increased T1 B cells and decreased marginal zone B cells in the spleen. Alcohol consumption also decreased mature B cells in the blood. It did not alter the chemotactic capacity of plasma to facilitate migration of splenocytes or the chemotactic response of splenocytes to CXCL13 and CCL21. However, the response of splenocytes to sphingosine-1-phosphate was impaired in alcohol-consuming, melanoma-bearing mice. The expression of sphingosine-1-phosphate receptor-1 (S1PR1) and sphingosine-1-phosphate lyase-1 (SPL1) in splenocytes was downregulated. Taken together, these results indicate that chronic alcohol consumption decreases peripheral blood B cells by compromising B cell egress from the spleen. The downregulation of S1PR1 and SPL1 expression in alcohol-consuming, melanoma-bearing mice could be associated with compromised egress of B cells from the spleen.
越来越多的研究表明 B 细胞参与抗肿瘤免疫。慢性酒精摄入与癌症患者生存率降低有关。酒精摄入对肿瘤宿主中 B 细胞的影响尚不清楚。在黑色素瘤荷瘤小鼠中的研究结果表明，慢性酒精摄入不会改变骨髓、脾脏和淋巴结中 B 细胞的百分比和数量，但会显著减少外周血中的 B 细胞。酒精摄入不会改变骨髓中 B 细胞的发育，也不会影响脾脏中的滤泡 B 细胞；然而，它增加了脾脏中的 T1 B 细胞并减少了边缘区 B 细胞。酒精摄入还会减少血液中的成熟 B 细胞。它不会改变血浆的趋化能力，以促进脾细胞的迁移，也不会影响脾细胞对 CXCL13 和 CCL21 的趋化反应。然而，在酒精摄入的黑色素瘤荷瘤小鼠中，脾细胞对鞘氨醇-1-磷酸的反应受损。脾细胞中鞘氨醇-1-磷酸受体-1（S1PR1）和鞘氨醇-1-磷酸裂解酶-1（SPL1）的表达下调。综上所述，这些结果表明，慢性酒精摄入通过损害 B 细胞从脾脏中的迁出而减少外周血 B 细胞。酒精摄入的黑色素瘤荷瘤小鼠中 S1PR1 和 SPL1 表达的下调可能与 B 细胞从脾脏迁出受损有关。
