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幽门螺杆菌外炎症蛋白A在大肠杆菌表面展示的暴露氨基酸序列的计算机模拟预测

In silico prediction of exposure amino acid sequences of outer inflammatory protein A of Helicobacter pylori for surface display on Eschierchia coli.

作者信息

Teymournejad Omid, Mobarez Ashraf M, Hassan Zuhair M, Moazzeni Seyed M, Yakhchali Bagher, Eskandari Vajihe

机构信息

Department of Bacteriology, Tarbiat Modares University, Tehran, Iran.

出版信息

Indian J Hum Genet. 2012 Jan;18(1):83-6. doi: 10.4103/0971-6866.96659.

DOI:10.4103/0971-6866.96659
PMID:22754227
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3385185/
Abstract

BACKGROUND

Outer inflammatory protein A (OipA) is an outer membrane protein of Helicobacter pylori that is involved in inducing IL-8 and intracellular signaling. In this study, we have predicted exposure amino acid sequences of OipA for insertion in permissive sites of CstH subunit of Eschierchia coli CS3 pilli for bacterial surface display.

DATABASES

National Center for Biotechnology Institute and Protein Data Bank. Servers: PHD, SABLE, GOR 4, SignalP3.0, TBBpred, PRODIV-TMHMM, TMRPres2D, CPH Models, PHYRE, GETAREA, VADAR, Pep state and pep window. Software: Swiss PDB viewer and Discovery studio.

RESULTS

In silico prediction of exposure amino acid sequences of OipA led to detection of six sequences of amino acid, 76-87, 106-112, 170-182, 222-230, 242-258, and 278-290. These sequences inserted between amino acid sequences 66-67, 100-101, and 109-110 of CstH that were predicted by Eskandari et al. as permissive sites of CstH.

CONCLUSION

OipA has the ability to induce IL-8 from gastric epithelial cells and some papers are mentioned that this outer membrane protein involve to attachment and intracellular signaling. Receptor of OipA and adhesion motifs on this protein is unknown. Detection of exposure motifs aids to recognition of adhesion motifs and receptor of OipA on gastric epithelial cells. In this study, we have predicted exposure amino acid sequences for insert to subunit CstH of CS3 pilli E. coli for surface display.

摘要

背景

外炎性蛋白A（OipA）是幽门螺杆菌的一种外膜蛋白，参与诱导白细胞介素-8和细胞内信号传导。在本研究中，我们预测了OipA的暴露氨基酸序列，以便插入大肠杆菌CS3菌毛CstH亚基的允许位点进行细菌表面展示。

数据库

美国国立生物技术信息中心和蛋白质数据库。服务器：PHD、SABLE、GOR 4、SignalP3.0-TBBpred、PRODIV-TMHMM、TMRPres2D、CPH模型、PHYRE、GETAREA、VADAR、Pep状态和Pep窗口。软件：Swiss PDB viewer和Discovery studio。

结果

对OipA暴露氨基酸序列的计算机模拟预测导致检测到六个氨基酸序列，即76 - 87、106 - 112、170 - 182、222 - 230、242 - 258和278 - 290。这些序列插入到Eskandari等人预测的CstH的66 - 67、100 - 101和109 - 110氨基酸序列之间，这些位点是CstH的允许位点。

结论

OipA有能力从胃上皮细胞诱导白细胞介素-8，一些文献提到这种外膜蛋白参与黏附和细胞内信号传导。OipA的受体及其蛋白上黏附基序尚不清楚。检测暴露基序有助于识别胃上皮细胞上OipA的黏附基序和受体。在本研究中，我们预测了用于插入大肠杆菌CS3菌毛CstH亚基进行表面展示的暴露氨基酸序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/e10f9b50df7c/IJHG-18-83-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/db00d1f0b61f/IJHG-18-83-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/2a849045c1a7/IJHG-18-83-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/5d0da2efef73/IJHG-18-83-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/549cd5d35439/IJHG-18-83-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/be8cb1089d13/IJHG-18-83-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/32536832ee64/IJHG-18-83-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/997c543e4428/IJHG-18-83-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/6de329b21470/IJHG-18-83-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/c4a0fad594df/IJHG-18-83-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/e10f9b50df7c/IJHG-18-83-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/db00d1f0b61f/IJHG-18-83-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/2a849045c1a7/IJHG-18-83-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/5d0da2efef73/IJHG-18-83-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/549cd5d35439/IJHG-18-83-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/be8cb1089d13/IJHG-18-83-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/32536832ee64/IJHG-18-83-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/997c543e4428/IJHG-18-83-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/6de329b21470/IJHG-18-83-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/c4a0fad594df/IJHG-18-83-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1d/3385185/e10f9b50df7c/IJHG-18-83-g010.jpg

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