重组幽门螺杆菌外膜蛋白H（HopH）对BALB/c小鼠外周巨噬细胞一氧化氮产生的影响。
Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice.
作者信息
Navidinia Masoumeh, Soleimani Neda, Bodagh Abadi Narges
机构信息
Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Departments of Microbiology and Microbial Biotechnology and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
出版信息
Avicenna J Med Biotechnol. 2019 Jul-Sep;11(3):229-233.
BACKGROUND
Some products of bacteria are reported as an immunomodulator. The () outer membrane proteins play an important role in stimulation of immune system. The present study was performed to determine the effect of recombinant HopH of on Nitric Oxide (NO) production and viability of mouse peritoneal macrophages.
METHODS
recombinant HopH was produced in this study. Mice peritoneal macrophages were purified and cultured. Different concentrations of recombinant HopH were used for stimulation of macrophages in order to evaluate NO production. The cell viability was detected by MTT assay. NO amounts released in to the supernatants of cultured macrophages and LPS-stimulated macrophages (10 ) were detected by Griess reagent.
RESULTS
Results demonstrated that the suppressive effect of high concentrations of recombinant HopH on NO release and the stimulation effect of protein was shown in 15 , compared to the control group. NO stimulation was significant in all the concentrations of LPS stimulated with HopH groups.
CONCLUSION
According to our findings, recombinant HopH has a toxic effect in high concentration on cell. So it can be an anticancer candidate.
背景
一些细菌产物被报道为免疫调节剂。（）外膜蛋白在刺激免疫系统中起重要作用。本研究旨在确定重组HopH对小鼠腹腔巨噬细胞一氧化氮（NO）产生和活力的影响。
方法
本研究制备了重组HopH。纯化并培养小鼠腹腔巨噬细胞。使用不同浓度的重组HopH刺激巨噬细胞以评估NO的产生。通过MTT法检测细胞活力。用Griess试剂检测培养的巨噬细胞和LPS刺激的巨噬细胞（10）上清液中释放的NO量。
结果
结果表明，与对照组相比，高浓度重组HopH对NO释放有抑制作用，在15时显示出蛋白质的刺激作用。在用HopH刺激的所有LPS浓度组中，NO刺激均显著。
结论
根据我们的研究结果，重组HopH在高浓度时对细胞有毒性作用。因此它可以作为一种抗癌候选物。
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