Liu Guo-Ling, Guo Hong-Hui, Sun Yuan-Ming
Department of Food Science, Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, China.
College of Food, South China Agricultural University, Guangzhou 51000, China.
Int J Mol Sci. 2012;13(5):6292-6302. doi: 10.3390/ijms13056292. Epub 2012 May 22.
Anthocyanins are naturally occurring polyphenols that impart bright color to fruits, vegetables and plants. In this study, the extraction of anthocyanins from freeze-dried fruit skin of downy rose-myrtle (Rhodomyrtus tomentosa (Ait.) Hassk var. Gangren) was optimized using response surface methodology (RSM). Using 60% ethanol containing 0.1% (v/v) hydrochloric acid as extraction solvent, the optimal conditions for maximum yields of anthocyanin (4.358 ± 0.045 mg/g) were 15.7:1 (v/w) liquid to solid ratio, 64.38 °C with a 116.88 min extraction time. The results showed good fits with the proposed model for the anthocyanin extraction (R(2) = 0.9944). Furthermore, the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) analysis of the anthocyanins extracted from the fruit skin of downy rose-myrtle revealed the presence of five anthocyanin components, which were tentatively identified as delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, petunidin-3-glucoside and malvidin-3-glucoside.
花青素是一种天然存在的多酚类物质，能赋予水果、蔬菜和植物鲜艳的颜色。在本研究中，采用响应面法（RSM）对绒毛桃金娘（Rhodomyrtus tomentosa (Ait.) Hassk var. Gangren）冻干果皮中花青素的提取工艺进行了优化。以含0.1%（v/v）盐酸的60%乙醇为提取溶剂，获得花青素最大产量（4.358±0.045 mg/g）的最佳条件为液固比15.7:1（v/w）、温度64.38℃、提取时间116.88分钟。结果表明，所建立的花青素提取模型拟合良好（R(2)=0.9944）。此外，对绒毛桃金娘果皮中提取的花青素进行高效液相色谱-电喷雾电离-质谱（HPLC-ESI-MS）分析的结果显示，存在五种花青素成分，初步鉴定为飞燕草素-3-葡萄糖苷、矢车菊素-3-葡萄糖苷、芍药素-3-葡萄糖苷、矮牵牛素-3-葡萄糖苷和锦葵素-3-葡萄糖苷。
