Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-sen University (Northern Campus), Guangzhou, China.
J Food Sci. 2012 Jan;77(1):C46-50. doi: 10.1111/j.1750-3841.2011.02447.x. Epub 2011 Nov 10.
Anthocyanins are naturally occurring compounds that impart color to fruits, vegetables, and plants. This study aims to optimize the microwave-assisted extraction (MAE) conditions of anthocyanins from mulberry (M. atropurpurea Roxb.) using response surface methodology (RSM). A Box-Behnken experiment was employed in this regard. Methanol concentration, microwave power, and extraction time were chosen as independent variables. The optimized conditions of MAE were as follows: 59.6% acidified methanol, 425 W power, 25 (v/w) liquid-to-solid ratio, and 132 s time. Under these conditions, 54.72 mg anthocyanins were obtained from 1.0 g mulberry powder. Furthermore, 8 anthocyanins were identified by high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) in mulberry extract. The results showed that cyanidin-3-glucoside and cyanidin-3-rutinoside are the major anthocyanins in mulberry. In addition, in comparison with conventional extraction, MAE is more rapid and efficient for extracting anthocyanins from mulberry.
花色苷是赋予水果、蔬菜和植物颜色的天然化合物。本研究旨在使用响应面法（RSM）优化从桑（Morus atropurpurea Roxb.）中提取花色苷的微波辅助提取（MAE）条件。在这方面采用了 Box-Behnken 实验。甲醇浓度、微波功率和提取时间被选为自变量。MAE 的优化条件如下：59.6%酸化甲醇、425 W 功率、25（v/w）液固比和 132 s 时间。在此条件下，从 1.0 g 桑粉中获得 54.72 mg 花色苷。此外，通过高效液相色谱-电喷雾电离质谱（HPLC-ESI-MS）在桑提取物中鉴定出 8 种花色苷。结果表明，矢车菊素-3-葡萄糖苷和矢车菊素-3-鼠李糖苷是桑中主要的花色苷。此外，与传统提取相比，MAE 更快速、高效地从桑中提取花色苷。
