J Thorac Dis. 2012 Jun 1;4(3):247-58. doi: 10.3978/j.issn.2072-1439.2012.06.02.
It has been 5 years since our team reported the first successful model of orthotopic single lung transplantation in the mouse. There has been great demand for this technique due to the obvious experimental advantages the mouse offers over other large and small animal models of lung transplantation. These include the availability of mouse-specific reagents as well as knockout and transgenic technology. Our laboratory has utilized this mouse model to study both immunological and non-immunological mechanisms of lung transplant physiology while others have focused on models of chronic rejection. It is surprising that despite our initial publication in 2007 only few other laboratories have published data using this model. This is likely due to the technical complexity of the surgical technique and perioperative complications, which can limit recipient survival. As two of the authors (XL and WL) have a combined experience of over 2500 left and right single lung transplants, this review will summarize their experience and delineate tips and tricks necessary for successful transplantation. We will also describe technical advances made since the original description of the model.
自我们团队报告首例成功的小鼠原位单肺移植模型以来，已经过去了 5 年。由于小鼠在肺移植的其他大、小动物模型中具有明显的实验优势，因此对这种技术的需求很大。这些优势包括可获得小鼠特异性试剂以及基因敲除和转基因技术。我们实验室利用这种小鼠模型研究了肺移植生理学的免疫和非免疫机制，而其他实验室则专注于慢性排斥反应模型。令人惊讶的是，尽管我们在 2007 年首次发表了该模型，但只有少数其他实验室使用该模型发表了数据。这可能是由于手术技术的复杂性和围手术期并发症，这可能会限制受体的存活率。作为两位作者（XL 和 WL）在左、右单肺移植方面拥有超过 2500 例的综合经验，这篇综述将总结他们的经验，并阐述成功移植所必需的技巧和窍门。我们还将描述自模型最初描述以来所取得的技术进展。
