Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department CHROMETA, KU Leuven, 3000 Leuven, Belgium.
Department of Thoracic Surgery, University Hospitals Leuven, 3000 Leuven, Belgium.
Cells. 2022 Jan 30;11(3):480. doi: 10.3390/cells11030480.
Lung transplantation improves the outcome and quality of life of patients with end-stage pulmonary disease. However, the procedure is still hampered by the lack of suitable donors, the complexity of the surgery, and the risk of developing chronic lung allograft dysfunction. Over the past decades, translational experiments in animal models have led to a better understanding of physiology and immunopathology following the lung transplant procedure. Small animal models (e.g., rats and mice) are mostly used in experiments regarding immunology and pathobiology and are preferred over large animal models due to the ethical aspects, the cost-benefit balance, and the high throughput possibility. In this comprehensive review, we summarize the reported surgical techniques for lung transplantation in rodent models and the management of perioperative complications. Furthermore, we propose a guide to help identify the appropriate species for a given experiment and discuss recent experimental findings in small animal lung transplant models.
肺移植改善了终末期肺部疾病患者的预后和生活质量。然而，该手术仍受到合适供体缺乏、手术复杂性和慢性肺移植物功能障碍发展风险的阻碍。在过去的几十年中，动物模型中的转化实验使人们对肺移植手术后的生理学和免疫病理学有了更好的理解。小动物模型（如大鼠和小鼠）主要用于免疫学和病理生物学实验，由于伦理方面、成本效益平衡和高通量的可能性，它们比大动物模型更受欢迎。在这篇全面的综述中，我们总结了啮齿动物模型中肺移植的报告手术技术和围手术期并发症的管理。此外，我们提出了一个指南，以帮助确定给定实验的合适物种，并讨论了小动物肺移植模型中的最新实验结果。
