橄榄苦苷对实验性大鼠脊髓损伤的抗炎作用
Anti-inflammatory effect of oleuropein in experimental rat spinal cord trauma.
作者信息
Khalatbary A R, Zarrinjoei Gh R
机构信息
Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Khazar Boulevard, Sari, Iran.
出版信息
Iran Red Crescent Med J. 2012 Apr;14(4):229-34. Epub 2012 Apr 1.
BACKGROUND
Spinal cord injury stimulates an inflammatory reaction that causes substantial secondary damage inside the injured spinal tissue. The purpose of this study was to determine the anti-inflammatory effect of oleuropein on traumatized spinal cord.
METHODS
Rats were randomly divided into four groups of 7 rats each as follows: Sham-operated group, trauma group, and oleuropein treatment groups (20 mg/kg, ip, immediately and 1 hour after spinal cord injury). Spinal cord samples were taken 24 hours after injury and studied for immunohistochemistry of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), nitrotyrosine, inducible nitricoxide synthase (iNOS), cyclooxygenase-2 (COX-2), and poly(ADP-ribose) polymerase (PARP).
RESULTS
Attenuated TNF-α, IL-1ß, nitrotyrosine, iNOS, COX-2, and PARP expression could be detected in the oleuropein-treated rats.
CONCLUSION
Oleuropein modulates inflammatory reactions following spinal cord injury.
背景
脊髓损伤会引发炎症反应，导致受损脊髓组织内出现大量继发性损伤。本研究的目的是确定橄榄苦苷对创伤性脊髓的抗炎作用。
方法
将大鼠随机分为四组，每组7只，如下所示：假手术组、创伤组和橄榄苦苷治疗组（脊髓损伤后立即及1小时腹腔注射20mg/kg）。损伤后24小时采集脊髓样本，进行肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、硝基酪氨酸、诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和聚（ADP-核糖）聚合酶（PARP）的免疫组织化学研究。
结果
在接受橄榄苦苷治疗的大鼠中可检测到TNF-α、IL-1β、硝基酪氨酸、iNOS、COX-2和PARP表达减弱。
结论
橄榄苦苷可调节脊髓损伤后的炎症反应。
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