Wu Zhijiao, Zhao Hong, Xue Ying, He Yujian, Li Xiangjun, Yuan Zhuobin
College of Chemistry and Chemical Engineering, Graduate University of Chinese, Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
J Nanosci Nanotechnol. 2012 Mar;12(3):2412-6. doi: 10.1166/jnn.2012.5787.
A rapid, simple and sensitive colorimetric detection method for melamine was proposed based on pyridine-3-boronic acid (PBA) modified gold nanoparticles (AuNPs). The formation of supramolecular hydrogen-bonded structure between PBA and melamine resulted in the aggregation of PBA modified AuNPs and the color change from red to blue. Melamine could be detected by colorimetric response of AuNPs that could be detected by naked eyes or a UV-vis spectrophotometer. The detection concentration of melamine ranged from 6.0 x 10(-8) to 1.6 x 10(-6) M, and the detection limit (3sigma) was 3.0 x 10(-8) M (i.e., 3.2 ppb). This provided an effective and facile colorimetric sensor for real-time and on-site determination of melamine. Particularly, the proposed method could be used to detect melamine in pretreated liquid milk products with high sensitivity and low interference, and the recoveries were from 95% to 102%.
基于吡啶 - 3 - 硼酸（PBA）修饰的金纳米粒子（AuNPs），提出了一种快速、简单且灵敏的三聚氰胺比色检测方法。PBA与三聚氰胺之间形成超分子氢键结构，导致PBA修饰的AuNPs聚集，颜色从红色变为蓝色。三聚氰胺可通过AuNPs的比色响应进行检测，该响应可用肉眼或紫外可见分光光度计检测。三聚氰胺的检测浓度范围为6.0×10⁻⁸至1.6×10⁻⁶ M，检测限（3σ）为3.0×10⁻⁸ M（即3.2 ppb）。这为实时、现场测定三聚氰胺提供了一种有效且简便的比色传感器。特别地，所提出的方法可用于高灵敏度、低干扰地检测预处理液态奶制品中的三聚氰胺，回收率为95%至102%。
