基于甲烷菌素介导的金纳米粒子合成的三聚氰胺比色检测。
Colorimetric detection of melamine based on methanobactin-mediated synthesis of gold nanoparticles.
机构信息
Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China; State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
出版信息
Food Chem. 2015 May 1;174:473-9. doi: 10.1016/j.foodchem.2014.11.098. Epub 2014 Nov 23.
A simple and rapid field-portable colorimetric method for the detection of melamine in liquid milk was reported. Methanobactin (Mb) could reduce Au (III) to Au (0) and mediate the synthesis of gold nanoparticles (Au-NPs). Upon the addition of melamine, melamine interacted with oxazolone ring of Mb, which interrupted the formation of Au-NPs. Melamine could also stimulate the aggregation of formed Au-NPs. In this paper, these characteristics have been used to detect melamine in liquid milk by naked eyes observation with a detection limit of 5.56 × 10(-6)M (0.7 mg/kg). Further, the plasmon absorbance of the formed Au-NPs allowed the quantitative detection of melamine by UV-vis spectrometer. A linear correlation was existed between the absorbance and the melamine concentration ranging from 3.90 × 10(-7)M to 3.97 × 10(-6)M with a correlation coefficient of 0.9685. The detection limit (3σ) obtained by UV-vis spectrum was as low as 2.38 × 10(-7)M (i.e., 0.03 mg/kg).
本文报道了一种简单快速的现场便携式比色法，用于检测液态奶中的三聚氰胺。甲羟戊酸（Mb）可以将 Au（III）还原为 Au（0），并介导金纳米粒子（Au-NPs）的合成。加入三聚氰胺后，三聚氰胺与 Mb 的噁唑酮环相互作用，从而中断了 Au-NPs 的形成。三聚氰胺还可以刺激形成的 Au-NPs 的聚集。在本文中，这些特性已被用于通过肉眼观察检测液态奶中的三聚氰胺，检测限为 5.56×10^(-6)M（0.7mg/kg）。此外，形成的 Au-NPs 的等离子体吸收允许通过紫外-可见分光光度计定量检测三聚氰胺。吸收与三聚氰胺浓度之间存在线性相关性，范围从 3.90×10^(-7)M 到 3.97×10^(-6)M，相关系数为 0.9685。通过紫外-可见光谱获得的检测限（3σ）低至 2.38×10^(-7)M（即 0.03mg/kg）。
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