Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Fuzhou, Fujian 350108, China.
Talanta. 2010 Oct 15;82(5):1654-8. doi: 10.1016/j.talanta.2010.07.035. Epub 2010 Aug 4.
A simple, rapid, field-portable colorimetric method for the detection of melamine based on melamine-induced color change of label-free gold nanoparticles (Au NPs) was developed in this study. Melamine can induced the aggregation of Au NPs and results in the color change from wine-red to purple, which provided a platform for rapid and field-portable colorimetric detection of melamine. The proposed method can be used to detect melamine in liquid milk and infant formula with a detection limit of 1.0 and 4.2ppm, respectively, within 30min by naked eyes observation without the aid of any advanced instrument and the need of any complex pretreatment, and detect as low as 0.15ppm of melamine in liquid milk and 2.5ppm of melamine in infant formula with UV-vis-spectroscopy. The proposed method is promising for on-site screening of melamine adulterant in milk products.
本研究开发了一种简单、快速、现场便携的比色法，用于基于无标记金纳米粒子（Au NPs）的三聚氰胺诱导的颜色变化来检测三聚氰胺。三聚氰胺可以诱导 Au NPs 的聚集，导致颜色从酒红色变为紫色，为快速和现场便携的三聚氰胺比色检测提供了一个平台。该方法可用于检测液态奶和婴儿配方奶粉中的三聚氰胺，通过肉眼观察，无需任何先进仪器和复杂的预处理，在 30 分钟内检测到的最低限分别为 1.0 和 4.2ppm，通过紫外-可见光谱法检测到的最低限分别为 0.15ppm 的三聚氰胺和 2.5ppm 的三聚氰胺。该方法有望用于现场筛选乳制品中的三聚氰胺掺杂物。
