Bhattacharya Debalina, Samanta Saheli, Mukherjee Ananda, Santra Chitta Ranjan, Ghosh Amar N, Niyogi Swapan Kumar, Karmakar Parimal
Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata 700032, West Bengal, India.
J Nanosci Nanotechnol. 2012 Mar;12(3):2513-21. doi: 10.1166/jnn.2012.6148.
Antibacterial activity of silver nanoparticles coated with different functionalizing agents i.e., polyethylene glycol, tween 80 and sodium dodecyl sulphate were evaluated on both normal and multi-drug resistant strains of bacteria. Under the same reaction conditions, these functionalizing agents were added separately to coat silver nanoparticles. Among these, polyethylene glycol coated nanoparticles were most effective in killing all the bacterial strains which includes Escherichia coli DH5a, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and multi-drug resistant clinical isolates of Shigella spp. (flexneri, boydii, sohnea) and Vibrio cholerae. The minimum inhibitory concentration of polyethylene glycol coated silver nanoparticles was also less compared to the other two sets of nanoparticles. Consistence with that polyethylene glycol coated nanoparticles produced more intracellular reactive oxygen species in bacteria. Moreover, when human cell lines MCF7 and Chang Liver were incubated in presence of these nanoparticles for 18 h with same concentrations as used for bacteria, no toxicity was observed. But significant increase in cell killing was observed with longer incubation time. Thus our present investigation implicates the potential therapeutic use of silver nanoparticles as antibacterial agent particularly the polyethylene glycol coated one.
评估了用不同官能化剂（即聚乙二醇、吐温80和十二烷基硫酸钠）包覆的银纳米颗粒对正常细菌菌株和多重耐药细菌菌株的抗菌活性。在相同反应条件下，分别添加这些官能化剂来包覆银纳米颗粒。其中，聚乙二醇包覆的纳米颗粒在杀死所有细菌菌株方面最有效，这些菌株包括大肠杆菌DH5α、枯草芽孢杆菌、藤黄微球菌、金黄色葡萄球菌以及志贺氏菌属（福氏志贺菌、鲍氏志贺菌、宋内志贺菌）和霍乱弧菌的多重耐药临床分离株。与其他两组纳米颗粒相比，聚乙二醇包覆的银纳米颗粒的最低抑菌浓度也更低。与此一致的是，聚乙二醇包覆的纳米颗粒在细菌中产生了更多的细胞内活性氧。此外，当人类细胞系MCF7和Chang Liver细胞在与用于细菌相同浓度的这些纳米颗粒存在下孵育18小时时，未观察到毒性。但随着孵育时间延长，观察到细胞杀伤显著增加。因此，我们目前的研究表明银纳米颗粒，特别是聚乙二醇包覆的银纳米颗粒作为抗菌剂具有潜在的治疗用途。
