Zhang Ting, Qian Li, Tang Meng, Xue Yuying, Kong Lu, Zhang Shanshan, Pu Yuepu
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
J Nanosci Nanotechnol. 2012 Mar;12(3):2866-73. doi: 10.1166/jnn.2012.5763.
To evaluate the cytotoxicity and genotoxicity of L-glutamic acid (Glu) coated Fe2O3 nanoparticles (hereafter refer as Glu@MNPs) on Chinese Hamster Lung (CHL) cells using Trypan blue dye exclusion assay, Oxidative stress markers, Comet assay and micronucleus (MN) assay. Results showed a low cytotoxicity with an IC50 was 254.739 microg/ml 36 h post incubation period in CHL cells. Furthermore, Cell redox status is slightly disturbed: Glu@MNPs exposure cause reactive oxygen species production, glutathione depletion and inactivation of some antioxidant enzymes: glutathione reductase, superoxide dismutase, but not catalase. Moreover, no significant genotoxic response was observed in CHL cells over concentration ranges from 8 to 128 microg/mL for all exposure time periods. The results suggest that the Glu@MNPs show biocompatibility In Vitro.
使用台盼蓝染料排除法、氧化应激标志物、彗星试验和微核（MN）试验，评估L-谷氨酸（Glu）包被的Fe2O3纳米颗粒（以下简称Glu@MNPs）对中国仓鼠肺（CHL）细胞的细胞毒性和遗传毒性。结果显示细胞毒性较低，在CHL细胞中孵育36小时后的IC50为254.739微克/毫升。此外，细胞氧化还原状态略有紊乱：暴露于Glu@MNPs会导致活性氧产生、谷胱甘肽消耗以及一些抗氧化酶（谷胱甘肽还原酶、超氧化物歧化酶，但不包括过氧化氢酶）失活。而且，在所有暴露时间段内，CHL细胞在8至128微克/毫升的浓度范围内均未观察到明显的遗传毒性反应。结果表明，Glu@MNPs在体外表现出生物相容性。
