Wiencek K M, Klapes N A, Foegeding P M
Department of Microbiology, North Carolina State University, Raleigh 27695-7624.
Appl Environ Microbiol. 1990 Sep;56(9):2600-5. doi: 10.1128/aem.56.9.2600-2605.1990.
The hydrophobicities of spores and vegetative cells of several species of the genera Bacillus and Clostridium were measured by using the bacterial adherence to hexadecane assay and hydrophobic interaction chromatography. Although spore hydrophobicity varied among species and strains, the spores of each organism were more hydrophobic than the vegetative cells. The relative hydrophobicities determined by the two methods generally agreed. Sporulation media and conditions appeared to have little effect on spore hydrophobicity. However, exposure of spore suspensions to heat treatment caused a considerable increase in spore hydrophobicity. The hydrophobic nature of Bacillus and Clostridium spores suggests that hydrophobic interactions may play a role in the adhesion of these spores to surfaces.
利用细菌对十六烷的粘附试验和疏水相互作用色谱法,测定了芽孢杆菌属和梭菌属几种菌种的芽孢和营养细胞的疏水性。尽管不同菌种和菌株的芽孢疏水性有所不同,但每种生物体的芽孢都比营养细胞更疏水。通过两种方法测定的相对疏水性总体上是一致的。芽孢形成培养基和条件似乎对芽孢疏水性影响不大。然而,将芽孢悬浮液进行热处理会导致芽孢疏水性显著增加。芽孢杆菌属和梭菌属芽孢的疏水性质表明,疏水相互作用可能在这些芽孢与表面的粘附过程中发挥作用。