Biswas Utpal Kumar, Kumar Arun
Department of Biochemistry, College of Medicine & JNM Hospital, Kalyani.
J Pak Med Assoc. 2012 May;62(5):417-21.
To determine the serum levels of methylglyoxal (MG) and carbonic anhydrase (CA) activity in known cases of type-II diabetes mellitus with insulin resistance and to compare them with age-sex matched healthy controls.
Eightysix participants were enrolled for the present case-controlled study with ages ranging from 25 to 44 years. The analytes measured were fasting blood glucose, serum insulin, methylglyoxal and carbonic anhydrase assay by standard procedures. Further carbonic anhydrase activities were measured in erythrocytes haemolysate and the effect of methylglyoxal on erythrocyte membrane was studied. The effects of methylglyoxal on erythrocyte carbonic anhydrase activity and insulin reactivity were also measured.
The mean serum levels of insulin, fasting blood glucose, carbonic anhydrase activity, methylglyoxal and body mass index (BMI) was significantly higher (p < 0.001) in patients compared to the controls. The carbonic anhydrase activity was directly proportional to increasing blood glucose concentration. The haemolysate prepared from the erythrocytes of patients and controls also showed significantly higher carbonic anhydrase activities in patients than the controls. Increased carbonic anhydrase activity in a dose-dependent manner with methylglyoxal was also observed.
Insulin resistance is accompanied by increased activity of carbonic anhydrase which is significantly related to increasing methylglyoxal levels.
测定已知患有胰岛素抵抗的II型糖尿病患者血清中甲基乙二醛（MG）水平和碳酸酐酶（CA）活性，并与年龄和性别匹配的健康对照者进行比较。
86名年龄在25至44岁之间的参与者被纳入本病例对照研究。通过标准程序测量的分析物包括空腹血糖、血清胰岛素、甲基乙二醛和碳酸酐酶检测。进一步测量红细胞溶血产物中的碳酸酐酶活性，并研究甲基乙二醛对红细胞膜的影响。还测量了甲基乙二醛对红细胞碳酸酐酶活性和胰岛素反应性的影响。
与对照组相比，患者的胰岛素、空腹血糖、碳酸酐酶活性、甲基乙二醛和体重指数（BMI）的平均血清水平显著更高（p < 0.001）。碳酸酐酶活性与血糖浓度升高成正比。由患者和对照者的红细胞制备的溶血产物也显示患者的碳酸酐酶活性显著高于对照者。还观察到碳酸酐酶活性随甲基乙二醛呈剂量依赖性增加。
胰岛素抵抗伴随着碳酸酐酶活性增加，这与甲基乙二醛水平升高显著相关。
