癌症患者胞质红细胞碳酸酐酶和过氧化氢酶的评估：碳酸酐酶活性升高。

An evaluation of cytosolic erythrocyte carbonic anhydrase and catalase in carcinoma patients: an elevation of carbonic anhydrase activity.

作者信息

Ozensoy Ozen, Kockar Feray, Arslan Oktay, Isik Semra, Supuran Claudiu T, Lyon Malcolm

机构信息

Balikesir University Faculty of Science and Literature, Department of Chemistry, Soma Street, 10100 Balikesir, Turkey.

出版信息

Clin Biochem. 2006 Aug;39(8):804-9. doi: 10.1016/j.clinbiochem.2006.03.001. Epub 2006 Apr 19.

DOI:10.1016/j.clinbiochem.2006.03.001

PMID:16624271

Abstract

OBJECTIVES

The antioxidant enzyme catalase and the CO2/HCO3- exchange enzyme carbonic anhydrase are both present in significant amounts in the cytosol of erythrocytes. The aim of the present study was to investigate whether these erythrocyte enzyme activities are altered in patients who have carcinoma.

DESIGN AND METHODS

Cytosolic erythrocyte enzyme activities were measured in 108 cancer patients presenting with carcinoma at one of variable sites, prior to clinical treatment. These were compared with an age- and sex-matched control group of 31 healthy volunteers.

RESULTS

Mean (+/-SD) catalase activities did not differ significantly, i.e. 0.0035 (+/-0.0015) EU/ml in carcinoma patients vs. 0.0031 (+/-0.00075) EU/ml in controls. However, mean carbonic anhydrase activities of 204 (+/-91) EU/ml in the carcinoma patients were significantly higher than the 158 (+/-35) EU/ml in controls (P value of 0.0065).

CONCLUSION

Cytosolic erythrocyte carbonic anhydrase levels may warrant further investigation as a potential peripheral marker in cancer.

摘要

目的

抗氧化酶过氧化氢酶和CO2/HCO3-交换酶碳酸酐酶在红细胞胞质中均大量存在。本研究的目的是调查这些红细胞酶活性在癌症患者中是否发生改变。

设计与方法

在108例不同部位患有癌症的患者临床治疗前，测量其红细胞胞质酶活性。将这些患者与31名年龄和性别匹配的健康志愿者对照组进行比较。

结果

平均（±标准差）过氧化氢酶活性无显著差异，即癌症患者为0.0035（±0.0015）酶活力单位/毫升，对照组为0.0031（±0.00075）酶活力单位/毫升。然而，癌症患者的平均碳酸酐酶活性为204（±91）酶活力单位/毫升，显著高于对照组的158（±35）酶活力单位/毫升（P值为0.0065）。