Rae P A, Yip C C, Schimmer B P
Can J Physiol Pharmacol. 1979 Aug;57(8):819-24. doi: 10.1139/y79-125.
Cell lines derived from a Syrian hamster insulinoma have been cloned in agar and maintained continuously in culture in vitro for 1 1/2 years. The cell lines have average doubling times of 48 h, they have modal chromosome numbers between 38 and 39, and they retain the ability to form tumors when injected into Syrian hamsters. The cells do not produce immunoreactive insulin when grown in vitro (less than 0.5 ng/mg protein), but do produce immunoreactive insulin when grown in vivo as tumors (mean value from six determinations = 7.1 +/- 1.5 ng/mg protein).
从叙利亚仓鼠胰岛素瘤中获得的细胞系已在琼脂中克隆，并在体外连续培养了一年半。这些细胞系的平均倍增时间为48小时，其众数染色体数在38至39之间，当注射到叙利亚仓鼠体内时仍保留形成肿瘤的能力。这些细胞在体外生长时不产生免疫反应性胰岛素（低于0.5纳克/毫克蛋白质），但作为肿瘤在体内生长时会产生免疫反应性胰岛素（六次测定的平均值 = 7.1 +/- 1.5纳克/毫克蛋白质）。
