Ide H, Eizuru Y, Minamishima Y, Katsuki T
First Department of Surgery, Miyazaki Medical College, Japan.
Gastroenterol Jpn. 1988 Feb;23(1):61-7. doi: 10.1007/BF02918858.
Rat pancreatic beta cells were transformed by simian virus 40 (SV40). The established beta cell lines expressed tumor antigen specific to SV40 and retained the ability to produce immunoreactive insulin (IRI) even at a high passage level. One of 12 transformed beta cell lines, SV-PB1205, was further investigated in vitro and in vivo. Firstly, effects of drugs on replication of the pancreatic beta cells were examined in in vitro experiments. Various drugs increased the uptake of 3H-thymidine into the cells. Those included D-glucose, tetragastrin and secretin. However, such effect was not observed for glucagon and growth hormone. Secondly, the SV40-transformed pancreatic beta cells were transplanted into diabetic rats. This produced such improvement of plasma glucose level at least for 2 weeks. The Significance of those experiments was discussed.
大鼠胰腺β细胞被猿猴病毒40（SV40）转化。建立的β细胞系表达SV40特异性肿瘤抗原，即使在传代次数很高时仍保留产生免疫反应性胰岛素（IRI）的能力。对12个转化的β细胞系之一SV-PB1205进行了进一步的体外和体内研究。首先，在体外实验中检测了药物对胰腺β细胞复制的影响。各种药物增加了细胞对3H-胸腺嘧啶核苷的摄取。这些药物包括D-葡萄糖、胃泌素和促胰液素。然而，胰高血糖素和生长激素未观察到这种作用。其次，将SV40转化的胰腺β细胞移植到糖尿病大鼠体内。这至少在2周内使血糖水平得到改善。讨论了这些实验的意义。
