通过遗传编码光掩蔽酪氨酸实现哺乳动物细胞中天冬氨酸磷酸化的光控。
Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine.
机构信息
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom.
出版信息
J Am Chem Soc. 2012 Jul 25;134(29):11912-5. doi: 10.1021/ja3046958. Epub 2012 Jul 12.
PMID:22758385
Abstract
We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.
摘要
我们报告了首例在哺乳动物细胞中对蛋白质进行定点基因编码光解笼酪氨酸的方法。通过光解笼封闭 STAT1 中的 Tyr701，我们证明了在哺乳动物细胞中光控酪氨酸磷酸化和信号转导是可行的。
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