Differential expression of IGF1, IGFR1 and IGFBP3 in mandibular condylar cartilage between male and female rats applied with malocclusion.

This study was designed to investigate the expression differences of insulin-like growth factor-1 (IGF1), IGF type 1 receptor (IGFR1) and IGF-binding protein-3 (IGFBP3) in mandibular condylar cartilage between male and female rats with experimentally created malocclusion. A total of 40 male and 40 female rats were used, and malocclusion was created by moving the first molars mesially and the third molars distally in the experimental group. Animals were killed at the end of the second and fourth weeks. Haematoxylin and eosin (HE) staining was performed to monitor the changes in cartilage morphology and thickness. Immunohistochemistry and real-time PCR were used to detect the expression of IGF1, IGFR1 and IGFBP3. Osteoarthritis (OA)-like changes were observed in the experimental groups, with 2-week females showing larger OA-like regions than 2-week males (P < 0·05). Compared to their age- and sex-matched controls, both 2- and 4-week males in the experimental groups displayed increased cartilage thickness in the posterior regions (P < 0·05). Compared to their age- and sex-matched controls, the expression of IGF1 was lower in 2-week female group (P < 0·05), but higher in 4-week female, 2- and 4-week male experimental groups (P < 0.05). Similarly, the expression of IGFR1 was lower in 2-week female experimental group (P < 0.05), but higher in 2-week male experimental group (P < 0.05). The higher expression of IGFBP3 was observed in 2-week female, 2- and 4-week male experimental groups (P < 0·05). These results indicate that condylar cartilage from male and female rats respond differently to the malocclusion in early stage of OA, with more serious degeneration in females.