基于甲酸的 Bruker Biotyper 基质辅助激光解吸电离飞行时间质谱仪对酵母和棒状杆菌属的直接、板上检测。
Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
机构信息
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
J Clin Microbiol. 2012 Sep;50(9):3093-5. doi: 10.1128/JCM.01045-12. Epub 2012 Jul 3.
Abstract
An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.
摘要
采用甲酸的板上测试方法在布鲁克 Biotyper 基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱系统上进行了评估，使用了 90 株酵母和 78 株棒状杆菌种的分离株，分别有 95.6%和 81.1%的酵母和 96.1%和 92.3%的棒状杆菌分离株被正确鉴定到属和种水平。采用甲酸的板上方法产生的鉴定百分比与传统但更繁琐的管基提取相似。
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