Ganmei Hospital, Kunming Medical University, Kunming 650000, China.
Gastroenterol Res Pract. 2012;2012:560345. doi: 10.1155/2012/560345. Epub 2012 Jun 12.
Objective. To establish a standardized animal model for liver fibrosis with the same assessment criteria for liver fibrosis studies that have been established on a unified platform. Methods. The standardized liver fibrosis model was established using Sprague-Dawley (SD) rats that either received an intraperitoneal injection of carbon tetrachloride (CCl(4)) in small dosages or ingested an ethanol solution. Results. The definite corresponding rules among modeling of different weeks and corresponding serology indices as well as different pathological staging can be observed by modeling with small dosages and slow, individualized, and combined administrations. Conclusion. This method can be used for the standardized establishment of a liver fibrosis model in rats across 5 pathological stages, ranging from S0 to S4, with a high success rate (89.33%) and low death rate (17.3%) because of the application of multiple hypotoxic chemicals for modeling. We refer to the criteria of Histological Grading and Staging of Chronic Hepatitis for Fibrosis established by the 10th World Digestive Disease Academic Conference in Los Angeles in September 1994 (revised in November 2000).
目的。建立一种标准化的肝纤维化动物模型，其肝纤维化研究的评估标准与已在统一平台上建立的标准一致。方法。采用小剂量腹腔注射四氯化碳（CCl(4)）或给予乙醇溶液的方法建立标准化肝纤维化模型。结果。通过小剂量、缓慢、个体化、联合给药的方式建模，可以观察到不同周数建模与相应血清学指标以及不同病理分期之间的明确对应规则。结论。该方法可用于标准化建立大鼠肝纤维化模型，涵盖从 S0 到 S4 的 5 个病理阶段，成功率高（89.33%），死亡率低（17.3%），因为采用了多种低毒性化学物质进行建模。我们参考了 1994 年 9 月在洛杉矶举行的第 10 届世界消化疾病学术会议（2000 年 11 月修订）制定的《慢性肝炎纤维化的组织学分级和分期标准》。
