Limbu Manoj Hang, Lei Liu, Zhengyuan Cheng, Jing Liu, Xiaoyi Zhang, Pingsheng Chen
Department of Pathology and Pathophysiology, Medical College of Southeast University, Ding Jia Qiao, Nanjing 210009, China.
Patholog Res Int. 2017;2017:8260508. doi: 10.1155/2017/8260508. Epub 2017 Aug 1.
It is already a proven fact that there exists a relationship between CLD (chronic liver disease) and kidney disease but still there is no available combined animal model of liver and kidney fibrosis on the same animal. An animal model is one of the important research tools in the field of medical science because it is important to build a model that can simulate the disease condition so that the particular disease can be studied. Therefore, the aim of this study is to build a less expensive, less time consuming, and reproducible model of hepatorenal fibrosis on rats. We administered combined intraperitoneal injection of CCl (Carbon Tetrachloride) and BSA (Bovine Serum Albumin) on a female Wistar rats. At the end, the liver and kidney tissues were examined under microscope to see whether we were successful in establishing the model or not. The results show that liver fibrosis was marked but the changes on the kidneys were mild. In this study, we were able to induce significant fibrosis in the liver and early stages of fibrosis in the kidneys. The result also demonstrated that the addition of BSA conferred a liver protective effect against CCl induced hepatotoxicity, whereas combination of CCl and BSA proved to be detrimental for kidneys.
慢性肝病（CLD）与肾病之间存在关联，这已是一个被证实的事实，但目前仍没有在同一只动物身上建立起肝肾纤维化的联合动物模型。动物模型是医学领域重要的研究工具之一，因为构建一个能够模拟疾病状况的模型对于研究特定疾病至关重要。因此，本研究的目的是建立一种成本较低、耗时较短且可重复的大鼠肝肾纤维化模型。我们对雌性Wistar大鼠进行腹腔联合注射四氯化碳（CCl）和牛血清白蛋白（BSA）。最后，在显微镜下检查肝脏和肾脏组织，以确定我们是否成功建立了模型。结果显示，肝脏纤维化明显，但肾脏的变化较为轻微。在本研究中，我们能够在肝脏中诱导出显著的纤维化，并在肾脏中诱导出纤维化的早期阶段。结果还表明，添加BSA对CCl诱导的肝毒性具有肝脏保护作用，而CCl和BSA的组合对肾脏则具有损害作用。
