Richards Vincent P, Alvarez Andres J, Luce Amy R, Bedenbaugh Molly, Mitchell Mary Lyn, Burne Robert A, Nascimento Marcelle M
Department of Biological Sciences, College of Science, Clemson University, Clemson, South Carolina, USA
DMD Program, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Infect Immun. 2017 Jul 19;85(8). doi: 10.1128/IAI.00106-17. Print 2017 Aug.
The oral microbiota associated with the initiation and progression of dental caries has yet to be fully characterized. The Human Oral Microbe Identification Using Next-Generation Sequencing (HOMI) approach was used to analyze the microbiomes of site-specific supragingival dental plaques from children with different caries status. Fifty-five children (2 to 7 years of age) were assessed at baseline and at 12 months and grouped as caries free (CF), caries active with enamel lesions (CAE), and caries active with dentin carious lesions (CA). Plaque samples from caries-free tooth surfaces (PF) and from enamel carious lesions (PE) and dentin carious lesions (PD) were collected. 16S community profiles were obtained by HOMI, and 408 bacterial species and 84 genus probes were assigned. Plaque bacterial communities showed temporal stability, as there was no significant difference in beta diversity values between the baseline and 12-month samples. Irrespective of collection time points, the microbiomes of healthy tooth surfaces differed substantially from those found during caries activity. All pairwise comparisons of beta diversity values between groups were significantly different ( < 0.05), except for comparisons between the CA-PF, CAE-PE, and CA-PE groups. genus probe 4 and genus probe 2 were the most frequently detected taxa across the plaque groups, followed by , which was highly abundant in CF-PF. Well-known acidogenic/aciduric species such as , , , and were found almost exclusively in CA-PD. The microbiomes of supragingival dental plaque differ substantially among tooth surfaces and children of different caries activities. In support of the ecological nature of caries etiology, a steady transition in community species composition was observed with disease progression.
与龋齿发生和发展相关的口腔微生物群尚未得到充分表征。采用下一代测序技术进行人类口腔微生物鉴定（HOMI）方法，分析不同龋齿状态儿童特定部位龈上牙菌斑的微生物群落。在基线和12个月时对55名儿童（2至7岁）进行评估，并分为无龋（CF）、有釉质病变的龋活跃（CAE）和有牙本质龋病变的龋活跃（CA）组。收集无龋牙面（PF）、釉质龋病变（PE）和牙本质龋病变（PD）的菌斑样本。通过HOMI获得16S群落图谱，并确定了408种细菌和84种属探针。菌斑细菌群落显示出时间稳定性，因为基线样本和12个月样本之间的β多样性值没有显著差异。无论采集时间点如何，健康牙面的微生物群落与龋活跃期发现的微生物群落有很大差异。除CA-PF、CAE-PE和CA-PE组之间的比较外，各组之间β多样性值的所有成对比较均有显著差异（<0.05）。属探针4和属探针2是菌斑组中最常检测到的分类群，其次是在CF-PF中含量很高的[未提及的分类群]。常见的产酸/耐酸菌种，如[未提及的菌种名称]，几乎只在CA-PD中发现。龈上牙菌斑的微生物群在不同牙面和不同龋活动的儿童之间有很大差异。支持龋病病因的生态本质，随着疾病进展观察到群落物种组成的稳定转变。
