Gao Xiao-Wei, Zhao Xu-Dong, Li Wen-Jing, Zhou Xin, Liu Ying
Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumchi 830013, Xinjiang Uygur Autonomous Region, China.
Int J Ophthalmol. 2012;5(2):147-50. doi: 10.3980/j.issn.2222-3959.2012.02.06. Epub 2012 Apr 18.
To evaluate the effect of Collagen cross-linking on the prevention of melting in rabbit corneas after alkali burn.
Twenty New Zealand white rabbits were randomly divided into model control group and collagen cross-linking treatment group. The second group of rabbits received collagen cross linked treatment. Both groups were applied with antibiotic eye drops to prevent infection. The corneas were evaluated for melting, opacity, pathological and immunohistochemistry, record the changes when 28 days after the animals were killed.
In the control group, 6 out of 8 rabbits showed corneal melting after injury (14±4) days, while two corneal perforated. In collagen cross-linking treatment group, one rabbit showed corneal melting after injury 23 days, without corneal perforation; corneal dissolution rate between the two groups was significantly different (P <0.05). Pathological examination suggested that in the treatment group, mild corneal edema, mild damage to collagen fibers, inflammatory cell infiltration was significantly less than the control group. Immunohistochemistry showed that corneal collagen fibers arranged in neat rows in the control group.
Collagen cross-linking treatment not only can prevent and delay the corneal melting after alkali burn, but also can reduce the destruction of corneal collagen fibers and infiltration of inflammatory cells in the corneal tissue.
评估胶原交联对预防碱烧伤后兔角膜溶解的效果。
将20只新西兰白兔随机分为模型对照组和胶原交联治疗组。第二组兔接受胶原交联治疗。两组均应用抗生素滴眼液预防感染。处死动物28天后，对角膜进行溶解、混浊、病理及免疫组化评估，记录变化情况。
对照组8只兔中有6只在伤后（14±4）天出现角膜溶解，2只角膜穿孔。胶原交联治疗组有1只兔在伤后23天出现角膜溶解，无角膜穿孔；两组间角膜溶解率差异有统计学意义（P<0.05）。病理检查提示，治疗组角膜轻度水肿、胶原纤维损伤及炎性细胞浸润明显少于对照组。免疫组化显示，对照组角膜胶原纤维排列整齐。
胶原交联治疗不仅能预防和延缓碱烧伤后角膜溶解，还能减轻角膜组织中胶原纤维破坏及炎性细胞浸润。
