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一种在猪角膜器官培养模型中评估核黄素/紫外线A角膜光疗效果的检测系统。

An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model.

作者信息

Perazzi Anna, Gomiero Chiara, Corain Livio, Iacopetti Ilaria, Grisan Enrico, Lombardo Marco, Lombardo Giuseppe, Salvalaio Gianni, Contin Roberta, Patruno Marco, Martinello Tiziana, Peruffo Antonella

机构信息

Department of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, Italy.

Department of Comparative Biomedicine and Food Science, University of Padova, 35020 Legnaro, Italy.

出版信息

Animals (Basel). 2020 Apr 23;10(4):730. doi: 10.3390/ani10040730.

DOI:10.3390/ani10040730
PMID:32340101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7652214/
Abstract

The purpose of this study was to investigate the response of porcine corneal organ cultures to riboflavin/UV-A phototherapy in the injury healing of induced lesions. A porcine corneal organ culture model was established. Corneal alterations in the stroma were evaluated using an assay system, based on an automated image analysis method able to (i) localize the holes and gaps within the stroma and (ii) measure the brightness values in these patches. The analysis has been performed by dividing the corneal section in 24 regions of interest (ROIs) and integrating the data analysis with a "multi-aspect approach." Three group of corneas were analyzed: healthy, injured, and injured-and-treated. Our study revealed a significant effect of the riboflavin/UV-A phototherapy in the injury healing of porcine corneas after induced lesions. The injured corneas had significant differences of brightness values in comparison to treated ( < 0.00) and healthy ( < 0.001) corneas, whereas the treated and healthy corneas showed no significant difference ( = 0.995). Riboflavin/UV-A phototherapy shows a significant effect in restoring the brightness values of damaged corneas to the values of healthy corneas, suggesting treatment restores the injury healing of corneas after lesions. Our assay system may be compared to clinical diagnostic methods, such as optical coherence tomography (OCT) imaging, for in vivo damaged ocular structure investigations.

摘要

本研究的目的是调查猪角膜器官培养物对核黄素/紫外线A光疗在诱导损伤愈合中的反应。建立了猪角膜器官培养模型。基于一种能够（i）定位基质内的孔洞和间隙以及（ii）测量这些区域亮度值的自动图像分析方法的检测系统，对基质中的角膜变化进行评估。通过将角膜切片划分为24个感兴趣区域（ROI）并采用“多方面方法”整合数据分析来进行分析。分析了三组角膜：健康组、损伤组和损伤并治疗组。我们的研究表明，核黄素/紫外线A光疗对诱导损伤后猪角膜的损伤愈合有显著影响。与治疗组（<0.00）和健康组（<0.001）角膜相比，损伤组角膜的亮度值有显著差异，而治疗组和健康组角膜之间无显著差异（=0.995）。核黄素/紫外线A光疗在将受损角膜的亮度值恢复到健康角膜的值方面显示出显著效果，表明该治疗可恢复损伤后角膜的损伤愈合。我们的检测系统可与临床诊断方法（如光学相干断层扫描（OCT）成像）相比较，用于体内受损眼结构的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/8e1949781320/animals-10-00730-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/d5f0d0c0ed8f/animals-10-00730-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/e8265f7901f5/animals-10-00730-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/19cfdb9607df/animals-10-00730-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/6eda8893da7e/animals-10-00730-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/ef2f61a3acfb/animals-10-00730-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/adc2f32a8dca/animals-10-00730-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/4fd9aa9a5e6f/animals-10-00730-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/90dbdf045097/animals-10-00730-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/8e1949781320/animals-10-00730-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/d5f0d0c0ed8f/animals-10-00730-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/e8265f7901f5/animals-10-00730-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/19cfdb9607df/animals-10-00730-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/6eda8893da7e/animals-10-00730-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/ef2f61a3acfb/animals-10-00730-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/adc2f32a8dca/animals-10-00730-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/4fd9aa9a5e6f/animals-10-00730-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/90dbdf045097/animals-10-00730-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc09/7652214/8e1949781320/animals-10-00730-g009.jpg

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Corneal cross-linking (CXL)-A clinical study to evaluate CXL as a treatment in comparison with medical treatment for ulcerative keratitis in horses.
大气压冷氦等离子体如何影响碱损伤角膜的生物力学行为？
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角膜交联（CXL）——一项关于评估角膜交联作为马匹溃疡性角膜炎治疗方法与药物治疗相比的临床研究。
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