Villaescusa Alejandra, García-Sancho Mercedes, Rodríguez-Franco Fernando, Sainz Angel
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, Avenida, Puerta de Hierro s/n, 28040 Madrid, Spain.
Vet Immunol Immunopathol. 2012 Sep 15;149(1-2):126-31. doi: 10.1016/j.vetimm.2012.06.004. Epub 2012 Jun 12.
A longitudinal study of peripheral blood lymphocyte subsets from 7 to 15 months of age was performed in Beagle dogs employing a multiparametric flow cytometry. The data were compared with data obtained from adult Beagle dogs that were housed in the same animal facilities and that were subjected to the same controls during the 34 weeks of the study. Absolute counts of total lymphocytes and CD3+ T, CD3+CD4+ Th and CD21+ B lymphocytes decreased during the entire 34 weeks period of the study in the young dogs group. The same was observed with regard to the percentage of CD3+CD4+ Th lymphocytes and the CD4/CD8 ratio, while the percentage of CD3+CD8+ Tc lymphocytes increased from 7 to 15 months of age. These age-related changes found in lymphocyte subsets distribution of young dogs led to level the absolute and relative values of adult dog lymphocytes. The observations of this longitudinal study illustrate the changes related to maturation of lymphocyte subsets that occur during early life in Beagle dogs.
采用多参数流式细胞术对7至15月龄比格犬的外周血淋巴细胞亚群进行了纵向研究。将这些数据与来自饲养在同一动物设施中且在研究的34周内接受相同对照的成年比格犬的数据进行了比较。在幼龄犬组中，整个34周研究期间总淋巴细胞以及CD3⁺ T、CD3⁺CD4⁺ Th和CD21⁺ B淋巴细胞的绝对计数均下降。CD3⁺CD4⁺ Th淋巴细胞百分比和CD4/CD8比值也出现同样情况，而CD3⁺CD8⁺ Tc淋巴细胞百分比在7至15月龄期间有所增加。幼龄犬淋巴细胞亚群分布中发现的这些与年龄相关的变化导致成年犬淋巴细胞的绝对和相对值趋于平稳。这项纵向研究的观察结果说明了比格犬幼年期淋巴细胞亚群成熟过程中发生的相关变化。
