Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
J Invest Dermatol. 2012 Dec;132(12):2700-8. doi: 10.1038/jid.2012.202. Epub 2012 Jul 5.
Acne vulgaris is the most common disease of the pilosebaceous unit. The pathogenesis of this inflammatory disease is complex, involving increased sebum production and perifollicular inflammation. To identify effective agents for factors that induce acne vulgaris, we explored the pharmacological potential of epigallocatechin-3-gallate (EGCG), which has been widely investigated as an anti-proliferative and anti-inflammatory agent. In this study, we demonstrated that topical application of EGCG to rabbit auricles reduced the size of the sebaceous glands. When applied to cultured human SZ95 sebocytes, EGCG strongly suppressed cell proliferation and lipogenesis. These actions of EGCG were reproduced in IGF-I-differentiated SZ95 sebocytes. To investigate the anti-inflammatory potential of EGCG, we evaluated pro-inflammatory cytokine synthesis in IGF-I-differentiated SZ95 sebocytes and found that expression of IL-1, IL-6, and IL-8 was decreased. These results provide early evidence that EGCG is an effective candidate for acne therapy whose mechanisms of action in IGF-I-differentiated SZ95 sebocytes include the inhibition of lipogenesis and inflammation.
寻常痤疮是皮脂腺单位最常见的疾病。这种炎症性疾病的发病机制复杂，涉及皮脂分泌增加和毛囊周围炎症。为了确定诱导寻常痤疮的因素的有效药物，我们研究了表没食子儿茶素没食子酸酯（EGCG）的药理学潜力，EGCG 已被广泛研究为一种具有抗增殖和抗炎作用的物质。在这项研究中，我们证明了 EGCG 局部应用于兔耳可减少皮脂腺的大小。当应用于培养的人 SZ95 皮脂腺细胞时，EGCG 强烈抑制细胞增殖和脂肪生成。IGF-I 分化的 SZ95 皮脂腺细胞中重现了 EGCG 的这些作用。为了研究 EGCG 的抗炎潜力，我们评估了 IGF-I 分化的 SZ95 皮脂腺细胞中促炎细胞因子的合成，发现 IL-1、IL-6 和 IL-8 的表达减少。这些结果为 EGCG 是一种有效的痤疮治疗候选药物提供了早期证据，其在 IGF-I 分化的 SZ95 皮脂腺细胞中的作用机制包括抑制脂肪生成和炎症。
