Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9096, USA.
J Invest Dermatol. 2012 Dec;132(12):2770-9. doi: 10.1038/jid.2012.207. Epub 2012 Jul 5.
Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE+) (N=9), SLE subjects with DLE (DLE+SLE+) (N=10), DLE subjects without SLE (DLE+SLE-) (N=11), and healthy controls (N=11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N=18), DLE+SLE+ (N=17), DLE+SLE- (N=23), and healthy subjects (N=22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value<0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), double-stranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA, -ssDNA, and -SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE- subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE- subjects may be nonpathogenic.
系统性红斑狼疮 (SLE) 伴盘状红斑狼疮 (DLE) 的患者被报道病情较轻。为了验证这一观察结果，我们使用含有 98 种自身抗原的夹心阵列比较了无 DLE 的 SLE 患者（DLE-SLE+）（N=9）、有 DLE 的 SLE 患者（DLE+SLE+）（N=10）、无 SLE 的 DLE 患者（DLE+SLE-）（N=11）和健康对照者（N=11）的自身抗体谱。我们使用免疫测定法在 DLE-SLE+（N=18）、DLE+SLE+（N=17）、DLE+SLE-（N=23）和健康受试者（N=22）中验证了差异表达的自身抗体。阵列显示 15 种 IgG 自身抗体（10 种针对核抗原）和 4 种 IgM 自身抗体差异表达（q 值<0.05）。与所有其他组（包括 DLE+SLE+组）相比，DLE-SLE+患者的 IgG 自身抗体对双链 DNA（dsDNA）、单链 DNA（ssDNA）、双链 RNA（dsRNA）、组蛋白 H2A 和 H2B 以及 SS-A（52 kDa）的表达更高（P<0.05）。测量抗 dsDNA、-ssDNA 和-SS-A（52 kDa）IgG 自身抗体的免疫测定也显示出相似的趋势（P<0.05）。健康受试者和 DLE+SLE- 受试者的 IgG 自身抗体对 alpha beta 晶蛋白、脂多糖、热休克同源物 70 和桥粒芯糖蛋白 3 的表达高于 DLE+SLE+和 DLE-SLE+受试者。针对核抗原的 IgG 自身抗体的 IgG:IgM 比值从健康受试者到 DLE-SLE+受试者逐渐升高。总之，DLE+SLE+与 DLE-SLE+患者相比，针对核抗原的 IgG 自身抗体水平较低，提示 DLE 表明疾病严重程度较低。健康和 DLE+SLE- 受试者针对某些抗原的 IgM 自身抗体水平较高可能是非致病性的。
