Laboratory of Investigative Dermatology, Rockefeller University, New York, New York, USA.
J Invest Dermatol. 2012 Nov;132(11):2552-64. doi: 10.1038/jid.2012.184. Epub 2012 Jul 5.
Psoriasis is a complex disease with an expanding definition of its pathological features. We sought to expand/refine the psoriasis transcriptome using 85 paired lesional and non-lesional samples from a cohort of patients with moderate-to-severe psoriasis vulgaris who were not receiving active psoriasis therapy. This new analysis identified 4,175 probe sets (representing 2,725 unique known genes) as being differentially expressed in psoriasis lesions compared with matched biopsies of non-lesional skin when the following criteria were applied: >2-fold change and false discovery rate <0.05. These probe sets represent the largest and most comprehensive set of genes defining psoriasis at the molecular level and within the previously unidentified genes, a link to functional pathways associated with metabolic diseases/diabetes and to cardiovascular risk pathways is identified. In addition, we profiled the serum of moderate-to-severe psoriatics compared with healthy controls to assess the overlap of overexpressed lesional genes with overexpressed systemic proteins. We identified linkage of functional pathways in lesional skin associated with metabolic diseases/diabetes and cardiovascular risk with those pathways overexpressed in the serum, suggesting a potential linkage between altered gene transcription in the skin and comorbidities commonly seen in patients with moderate-to-severe psoriasis.
银屑病是一种复杂的疾病，其病理特征的定义不断扩大。我们使用来自一组未接受活性银屑病治疗的中度至重度寻常型银屑病患者的 85 对病变和非病变样本，旨在通过 85 对病变和非病变样本来扩展/完善银屑病转录组。当应用以下标准时，与匹配的非病变皮肤活检相比，该新分析确定了 4175 个探针集（代表 2725 个独特的已知基因）在银屑病病变中差异表达：>2 倍变化和错误发现率 <0.05。这些探针集代表了在分子水平上定义银屑病的最大和最全面的基因集，在以前未识别的基因中，与代谢疾病/糖尿病和心血管风险途径相关的功能途径的联系被确定。此外，我们对中度至重度银屑病患者的血清与健康对照进行了分析，以评估病变基因与过度表达的系统性蛋白之间的重叠。我们发现与代谢疾病/糖尿病和心血管风险相关的皮肤病变中的功能途径与血清中过度表达的途径之间存在联系，这表明皮肤中改变的基因转录与中度至重度银屑病患者常见的合并症之间存在潜在联系。
