Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701, Republic of Korea.
Analyst. 2012 Sep 7;137(17):3917-20. doi: 10.1039/c2an35458f. Epub 2012 Jul 5.
Complementary metal oxide semiconductor (CMOS) image sensor is a powerful tool for biosensing applications. In this present study, CMOS image sensor has been exploited for detecting glucose levels by simple photon count variation with high sensitivity. Various concentrations of glucose (100 mg dL(-1) to 1000 mg dL(-1)) were added onto a simple poly-dimethylsiloxane (PDMS) chip and the oxidation of glucose was catalyzed with the aid of an enzymatic reaction. Oxidized glucose produces a brown color with the help of chromogen during enzymatic reaction and the color density varies with the glucose concentration. Photons pass through the PDMS chip with varying color density and hit the sensor surface. Photon count was recognized by CMOS image sensor depending on the color density with respect to the glucose concentration and it was converted into digital form. By correlating the obtained digital results with glucose concentration it is possible to measure a wide range of blood glucose levels with great linearity based on CMOS image sensor and therefore this technique will promote a convenient point-of-care diagnosis.
互补金属氧化物半导体（CMOS）图像传感器是生物传感应用的强大工具。在本研究中，通过简单的光子计数变化实现了对葡萄糖水平的高灵敏度检测，利用 CMOS 图像传感器来检测葡萄糖水平。将不同浓度的葡萄糖（100 毫克/分升至 1000 毫克/分升）添加到简单的聚二甲基硅氧烷（PDMS）芯片上，并借助酶反应来催化葡萄糖的氧化。在酶反应过程中，氧化的葡萄糖在显色剂的帮助下产生棕色，颜色密度随葡萄糖浓度而变化。光子穿过具有不同颜色密度的 PDMS 芯片，并撞击传感器表面。CMOS 图像传感器根据颜色密度识别光子计数，将其转换为数字形式。通过将获得的数字结果与葡萄糖浓度相关联，基于 CMOS 图像传感器可以测量广泛的血糖水平，具有很好的线性度，因此该技术将促进便捷的即时诊断。
