将溶致变色荧光团转化为基于蛋白质的 pH 指示剂，用于极端酸性环境。
Converting a solvatochromic fluorophore into a protein-based pH indicator for extreme acidity.
机构信息
Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
出版信息
Angew Chem Int Ed Engl. 2012 Jul 27;51(31):7674-9. doi: 10.1002/anie.201204029. Epub 2012 Jul 4.
Live-cell pH measurements: An environment-sensitive fluorophore (green) was site-specifically introduced on HdeA, an acid-resistant chaperone showing pH-mediated conformational changes under low pH conditions. A survey of the attachment sites led to the discovery of one position on HdeA at which the attached fluorophore showed a strong fluorescence increase upon acidification.
活细胞 pH 值测量：一种环境敏感的荧光团（绿色）被特异性地引入到 HdeA 上，HdeA 是一种耐酸伴侣蛋白，在低 pH 条件下会发生 pH 介导的构象变化。对附着位点的调查导致在 HdeA 上发现了一个位置，在该位置上，附着的荧光团在酸化时会显示出强烈的荧光增强。
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