Zhu Li-Li, Gou Zheng-Jiang, Zeng Zhi-Hua, Zhuo Lian-Shi
College of Chinese Medicine, Chongqing Medical University, Chongqing 400016, China.
Zhen Ci Yan Jiu. 2012 Apr;37(2):99-103.
To observe the influence of electroacupuncture (EA) intervention on liver function and retinol binding protein 4 (RBP 4) in nonalcoholic fatty liver disease (NAFLD) rats, so as to study its mechanisms underlying improving liver function.
A total of 44 SD rats were randomly allocated to normal control, model, EA and non-acupoint groups, with 11 rats in each group. NAFLD model was established by feeding the rats with high glucose and high fat forage for 5 weeks. EA (2 Hz, 1.5 V, 15 min) was applied to unilateral "Fenglong" (ST 40), "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Taichong" (LR 3), once daily for 4 weeks. Serum RBP 4 content was assayed by enzyme-linked immunosorbent assay (ELISA), and serum glutamic-oxaloacetic transaminase (AST), alanine aminotransferase (ALT), total cholesterol (TC), and triglycerides (TG) contents were detected by chromatometry.
Compared with the normal control group, serum AST, ALT, TC, TG and RBP 4 contents in the model group were increased significantly (P<0. 01), while in comparison with the model group, the levels of the abovementioned 5 serum indexes were down-regulated considerably in the EA group (P < 0.05) rather than in the non-acupoint group (P > 0.05).
EA intervention can lower serum AST, ALT, TC, TG and RBP 4 contents in NAFLD rats, which may be responsible for its action in improving NAFLD in clinic.
观察电针干预对非酒精性脂肪性肝病（NAFLD）大鼠肝功能及视黄醇结合蛋白4（RBP 4）的影响，以探讨其改善肝功能的机制。
将44只SD大鼠随机分为正常对照组、模型组、电针组和非穴位组，每组11只。通过给予大鼠高糖高脂饲料喂养5周建立NAFLD模型。电针（2 Hz，1.5 V，15分钟）刺激单侧“丰隆”（ST 40）、“足三里”（ST 36）、“三阴交”（SP 6）和“太冲”（LR 3），每日1次，共4周。采用酶联免疫吸附测定（ELISA）法检测血清RBP 4含量，用比色法检测血清谷氨酸草酰乙酸转氨酶（AST）、丙氨酸转氨酶（ALT）、总胆固醇（TC）和甘油三酯（TG）含量。
与正常对照组比较，模型组血清AST、ALT、TC、TG和RBP 4含量显著升高（P<0.01）；与模型组比较，电针组上述5项血清指标水平明显下调（P<0.05），而非穴位组差异无统计学意义（P>0.05）。
电针干预可降低NAFLD大鼠血清AST、ALT、TC、TG和RBP 4含量，这可能是其临床改善NAFLD的作用机制。
