Feng Wen-Qi, Liu Qun-Ying, Zeng Zhi-Hua, Zhou Lian-Shi
School of Chinese Medicine, Chongqing Medical University, Chongqing 400050, China.
Zhen Ci Yan Jiu. 2009 Apr;34(2):89-92, 119.
To study the effect of electroacupuncture (EA) on liver cytochrome P450 1 A 1 immunoactivity, superoxide dismutase (SOD) and malondialdehyde (MDA) contents in nonalcoholic fatty liver disease (NAFLD) rats.
Thirty male SD rats were randomized into normal control (n = 10), model (n = 10) and EA (n = 10) groups. NAFLD model was established by feeding the animal with high-fat forage for 8 weeks. EA (1.6-2 Hz, 1-4 mA) was applied to bilateral "Zusanli" (ST 36), "Fenglong" (ST 40), "Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 15 min, once daily for 4 weeks. Then the rats anesthetized with ether were killed for collecting liver tissue. Following homogenate and centrifugalization of the partial liver tissue, the supernatant was collected for assaying superoxide dismutase (SOD) and malondialdehyde (MDA) contents by xanthinoxidase chromometry and thio-malonylurea chromometry respectively. The other partial liver tissue samples were fixed in 10% formalin, followed by paraffin imbedding and sectioning (4 microm), and staining with streptavidin-perosidase methods respectively for displaying hepatic pathological changes and cytochrom P450 1 A 1 immunoreaction.
Compared with control group, hepatic SOD content of model group was significantly lower (P < 0.05), and MDA level and cytochrome P450 1 A 1 (CYP 1 A 1) integrated optic density (IOD) value were obviously higher in model group (P < 0.05). In comparison with model group, liver SOD level of EA group increased considerably (P < 0.05), while MDA level and CYP 1 A 1 IOD of EA group decreased evidently in EA group (P < 0.05).
EA can effectively reduce lipid peroxidation and up-regulate CYP 1 A 1 expression in nonalcoholic fatty liver tissue, which may contribute to its effect in improving fatty liver.
研究电针（EA）对非酒精性脂肪性肝病（NAFLD）大鼠肝脏细胞色素P450 1A1免疫活性、超氧化物歧化酶（SOD）和丙二醛（MDA）含量的影响。
将30只雄性SD大鼠随机分为正常对照组（n = 10）、模型组（n = 10）和电针组（n = 10）。通过给动物喂食高脂饲料8周建立NAFLD模型。将电针（1.6 - 2Hz，1 - 4mA）施加于双侧“足三里”（ST 36）、“丰隆”（ST 40）、“三阴交”（SP 6）和“太冲”（LR 3），持续15分钟，每日1次，共4周。然后用乙醚麻醉大鼠并处死，收集肝脏组织。将部分肝脏组织匀浆并离心后，分别采用黄嘌呤氧化酶比色法和硫代巴比妥酸比色法测定上清液中SOD和MDA的含量。将另一部分肝脏组织样本用10%福尔马林固定，随后进行石蜡包埋、切片（4μm），分别采用链霉亲和素 - 过氧化物酶法染色，以显示肝脏病理变化和细胞色素P450 1A1免疫反应。
与对照组相比，模型组肝脏SOD含量显著降低（P < 0.05），模型组MDA水平和细胞色素P450 1A1（CYP 1A1）积分光密度（IOD）值明显升高（P < 0.05）。与模型组相比，电针组肝脏SOD水平显著升高（P < 0.05），而电针组MDA水平和CYP 1A1 IOD明显降低（P < 0.05）。
电针可有效降低非酒精性脂肪肝组织中的脂质过氧化作用并上调CYP 1A1表达，这可能有助于其改善脂肪肝的作用。
