Feng Wen-Qi, Zeng Zhi-Hua, Zhuo Lian-Shi
School of Chinese Medicine, Chongqing Medical University, Chongqing 400050, China.
Zhen Ci Yan Jiu. 2008 Apr;33(2):111-5.
To investigate the mechanism of electroacupuncture (EA) in treating nonalcoholic fatty liver disease (NAFLD) in rats.
Thirty male SD rats were randomized into normal control, NAFLD model and EA groups, with 10 cases in each. NAFLD model was established by feeding the rats with high-fat food for 8 weeks. EA (2 Hz, 4 mA) was applied to "Zusanli" (ST 36), "Fenglong" (ST 40), "Sanyinjiao" (SP 6), and "Taichong" (LR 3) for 15 min, once daily for 4 weeks. Fasting blood sugar (FBG) and serum free fatty acids (FFA) were detected with chromatometry; serum insulin (INS) was determined with radioimmunoassay; homeostasis model assessment insulin resistance (HOMA-IR) and liver index (LI) were calculated. Liver tissue was cut into sections for detecting pathological changes.
Compared with normal control group, FBG, INS, FFA, HOMA-IR and LI of model group increased significantly (P<0.05), and hepatic diffuse steatosis with cellular necrosis and inflammatory cell infiltration was obvious. In comparison with model group, all the 5 indexes in EA group decreased significantly (P<0.05), and pathological changes as hepatic steatosis, etc. were significantly reduced.
EA may be used to effectively improve NAFLD by lowering insulin resistance.
探讨电针治疗大鼠非酒精性脂肪性肝病（NAFLD）的机制。
将30只雄性SD大鼠随机分为正常对照组、NAFLD模型组和电针组，每组10只。通过给大鼠喂食高脂食物8周建立NAFLD模型。将电针（2Hz，4mA）应用于“足三里”（ST36）、“丰隆”（ST40）、“三阴交”（SP6）和“太冲”（LR3），持续15分钟，每天1次，共4周。采用色谱法检测空腹血糖（FBG）和血清游离脂肪酸（FFA）；采用放射免疫法测定血清胰岛素（INS）；计算稳态模型评估胰岛素抵抗（HOMA-IR）和肝指数（LI）。将肝组织切成切片以检测病理变化。
与正常对照组相比，模型组的FBG、INS、FFA、HOMA-IR和LI显著升高（P<0.05），肝弥漫性脂肪变性伴细胞坏死和炎性细胞浸润明显。与模型组相比，电针组的5项指标均显著降低（P<0.05），肝脂肪变性等病理变化明显减轻。
电针可能通过降低胰岛素抵抗有效改善NAFLD。
