粘质沙雷氏菌碱性磷酸酶同工酶的多种形式。

Multiple forms of alkaline phosphatase isoenzymes of Serratia marcescens.

作者信息

Bhatti A R, Ijaz M K, Chaudhry G R

机构信息

Department of Biochemistry, University of Sydney, N.S.W., Australia.

出版信息

FEMS Microbiol Lett. 1990 Sep 1;59(1-2):111-5. doi: 10.1111/j.1574-6968.1990.tb03807.x.

DOI:10.1111/j.1574-6968.1990.tb03807.x

PMID:2276602

Abstract

Alkaline phosphatase (APase) isoenzymes produced by different strains of Serratia marcescens were examined. Variation of isoenzyme patterns with respect to number and their mobilities in starch gels after electrophoresis were observed. Ten strains gave a 1-isoenzyme pattern with 5 different mobilities; 7 strains gave a 2-isoenzyme pattern with 3 different mobilities; 9 strains gave a 3-isoenzyme pattern with 5 different mobilities; and 3 strains gave a 4-isoenzyme pattern. Three strains synthesized two electrophoretically distinct APases in low phosphate medium. A high concentration of inorganic phosphate induced the synthesis of one of these APase isoenzymes.

摘要

对不同粘质沙雷氏菌菌株产生的碱性磷酸酶（APase）同工酶进行了检测。观察了同工酶谱在电泳后淀粉凝胶中的数量及其迁移率的变化。10个菌株呈现出具有5种不同迁移率的1种同工酶谱；7个菌株呈现出具有3种不同迁移率的2种同工酶谱；9个菌株呈现出具有5种不同迁移率的3种同工酶谱；3个菌株呈现出4种同工酶谱。3个菌株在低磷酸盐培养基中合成了两种电泳性质不同的APase。高浓度的无机磷酸盐诱导了其中一种APase同工酶的合成。