School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Dev Comp Immunol. 2012 Oct;38(2):215-23. doi: 10.1016/j.dci.2012.06.007. Epub 2012 Jul 2.
This study investigates the ontogeny of New Zealand groper (Polyprion oxygeneios) immune system, a new species for aquaculture in the Southern Pacific Ocean. In the eggs, both lysozyme and IgM were detected. Egg IgM was found at 1.07-1.56 μg/g wet weight and consisted of monomers compared to the polymerized IgM found in adult serum. In larvae, the head-kidney (HK) was first observed at 6 dph, followed by the spleen at 16 dph, and thymus at 20 dph, and within these organs IgM(+) cells were first detected in the HK (12 dph), then the spleen (32 dph) and finally in the thymus and the gastrointestinal tract (45 dph). Low levels of Igμ heavy chain transcripts were detected at 2 and 3 dph and they increased at 9 dph. Igμ expression further increased from day 45 onwards. In juveniles (115 dph), the HK and blood showed similar percentages of IgM(+) cells as the adult groper. These results highlight the important maturation steps that occur during the development of the immune system in the marine teleost P. oxygeneios.
本研究调查了新西兰石斑鱼（Polyprion oxygeneios）免疫系统的个体发生，这是一种在南太平洋地区新的水产养殖物种。在卵中，均检测到溶菌酶和 IgM。卵 IgM 含量为 1.07-1.56μg/g 湿重，与成年血清中发现的聚合 IgM 相比为单体。在幼虫中，头肾（HK）在 6 dph 首次被观察到，随后在 16 dph 出现脾脏，在 20 dph 出现胸腺，在这些器官中，HK（12 dph）、脾脏（32 dph）和最后在胸腺和胃肠道（45 dph）中首次检测到 IgM(+)细胞。在 2 天和 3 天检测到低水平的 Igμ 重链转录物，它们在 9 天增加。Igμ 的表达从第 45 天开始进一步增加。在幼鱼（115 dph）中，HK 和血液中的 IgM(+)细胞百分比与成年石斑鱼相似。这些结果突出了海洋硬骨鱼 P. oxygeneios 免疫系统发育过程中的重要成熟步骤。
