Anderson Sally A, Hulston Debbie A, McVeagh S Margaret, Webb Victoria L, Smith Peter J
National Institute of Water and Atmospheric Research (NIWA) Ltd, Private Bag 14-901, Wellington 6241, New Zealand.
J Microbiol Methods. 2009 Oct;79(1):62-6. doi: 10.1016/j.mimet.2009.07.022. Epub 2009 Aug 5.
An in vitro culture method was developed for the ciliated protozoa Uronema marinum isolated from New Zealand aquacultured groper (Polyprion oxygeneios). Both formulated media and sterile seawater supplemented with homogenised fish tissue as a food source supported growth of U. marinum achieving cell densities of up to 1 x 10(5)cells/mL in culture. A cryopreservation method based on a cryomix formula of 20% glycerol, 10% fetal bovine serum and 70% cultured U. marinum, incorporating a slow freeze method to -80 degrees C, then liquid nitrogen storage, allowed cryogenic storage of cells and successful re-culture up to 12 months in storage.
已开发出一种体外培养方法，用于培养从新西兰水产养殖的鞍带石斑鱼（波纹唇鱼）中分离出的纤毛原生动物海产尾丝虫。配方培养基和添加了匀浆鱼组织作为食物来源的无菌海水均能支持海产尾丝虫的生长，在培养中细胞密度可达1×10⁵个细胞/毫升。一种基于由20%甘油、10%胎牛血清和70%培养的海产尾丝虫组成的冷冻混合物配方的冷冻保存方法，采用慢速冷冻至-80℃，然后液氮保存，可实现细胞的低温保存，并在保存长达12个月后成功再培养。
