Department of Orthopaedic Surgery, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Eur Spine J. 2012 Sep;21(9):1734-9. doi: 10.1007/s00586-012-2418-5. Epub 2012 Jul 6.
Implantation of TheraCyte 4 × 10(6) live parathyroid cells can increase the bone marrow density of the spine of ovariectomized rats. There has been no published study examining the effect of such implantation on spinal fusion outcomes. The purpose of this study was to examine the effect of TheraCyte-encapsulated parathyroid cells on posterolateral lumbar fusions in a rat model.
Forty Sprague-Dawley rats underwent single-level, intertransverse process spinal fusions using iliac crest autograft. The rats were randomly assigned to two groups: Group 1 rats received sham operations on their necks (control; N = 20); Group 2 rats were implanted with TheraCyte-encapsulated 4 × 10(6) live parathyroid cells into the subcutis of their necks (TheraCyte; N = 20). Six weeks after surgery the rats were killed. Fusion was assessed by inspection, manual palpation, radiography, and histology. Blood was drawn to measure the serum levels of calcium, phosphorus, and intact parathyroid hormone (iPTH).
Based on manual palpation, the control group had a fusion rate of 33 % (6/18) and the TheraCyte group had a fusion rate of 72 % (13/18) (P = 0.044). Histology confirmed the manual palpation results. Serum iPTH levels were significantly higher in the TheraCyte group compared with the control group (P < 0.05); neither serum calcium nor phosphorus levels were significantly different between the two groups.
This pilot animal study revealed that there were more fusions in rats that received TheraCyte-encapsulated 4 × 10(6) live parathyroid cells than in control rats without significant change in serum calcium or phosphorus concentrations. As with any animal study, the results may not extrapolate to a higher species. Further studies are needed to determine if these effects are clinically significant.
植入 4×10(6)个活甲状旁腺细胞的 TheraCyte 可以增加去卵巢大鼠的骨髓密度。目前还没有发表的研究检查这种植入对脊柱融合结果的影响。本研究的目的是检查 TheraCyte 包裹的甲状旁腺细胞对大鼠模型后路腰椎融合的影响。
40 只 Sprague-Dawley 大鼠接受单节段、横突间脊柱融合术，使用髂嵴自体移植物。大鼠被随机分为两组：第 1 组大鼠颈部接受假手术（对照组；N=20）；第 2 组大鼠颈部皮下植入 TheraCyte 包裹的 4×10(6)个活甲状旁腺细胞（TheraCyte；N=20）。手术后 6 周，大鼠被处死。通过检查、手动触诊、放射学和组织学评估融合情况。抽取血液测量血清钙、磷和完整甲状旁腺激素（iPTH）水平。
根据手动触诊，对照组融合率为 33%（6/18），TheraCyte 组融合率为 72%（13/18）（P=0.044）。组织学证实了手动触诊结果。TheraCyte 组血清 iPTH 水平明显高于对照组（P<0.05）；两组血清钙或磷水平无显著差异。
这项初步动物研究表明，接受 TheraCyte 包裹的 4×10(6)个活甲状旁腺细胞的大鼠融合更多，而对照组大鼠无明显血清钙或磷浓度变化。与任何动物研究一样，结果可能不适用于更高的物种。需要进一步的研究来确定这些影响是否具有临床意义。
