State Key Laboratory for Agrobiotechnology and MOE Key Laboratory of Crop Heterosis and Utilization and MOA Key Laboratory of Crop Genomics and Genetic Improvement, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.
Genomics Proteomics Bioinformatics. 2012 Apr;10(2):94-106. doi: 10.1016/j.gpb.2012.05.002. Epub 2012 Jun 9.
Powdery mildew (Pm) caused by the infection of Blumeria graminis f. sp. tritici (Bgt) is a worldwide crop disease resulting in significant loss of wheat yield. To profile the genes and pathways responding to the Bgt infection, here, using Affymetrix wheat microarrays, we compared the leaf transcriptomes before and after Bgt inoculation in two wheat genotypes, a Pm-susceptible cultivar Jingdong 8 (S) and its near-isogenic line (R) carrying a single Pm resistant gene Pm30. Our analysis showed that the original gene expression status in the S and R genotypes of wheat was almost identical before Bgt inoculation, since only 60 genes exhibited differential expression by P = 0.01 cutoff. However, 12 h after Bgt inoculation, 3014 and 2800 genes in the S and R genotype, respectively, responded to infection. A wide range of pathways were involved, including cell wall fortification, flavonoid biosynthesis and metabolic processes. Furthermore, for the first time, we show that sense-antisense pair genes might be participants in wheat-powdery mildew interaction. In addition, the results of qRT-PCR analysis on several candidate genes were consistent with the microarray data in their expression patterns. In summary, this study reveals leaf transcriptome changes before and after powdery mildew infection in wheat near-isogenic lines, suggesting that powdery mildew resistance is a highly complex systematic response involving a large amount of gene regulation.
小麦白粉病由禾本科布氏白粉菌（Bgt）感染引起，是一种世界性作物病害，可导致小麦产量的重大损失。为了研究响应 Bgt 感染的基因和途径，我们使用 Affymetrix 小麦微阵列，在两个小麦基因型（易感品种 Jingdong 8（S）及其携带单个 Pm 抗性基因 Pm30 的近等基因系（R））中，比较了 Bgt 接种前后的叶片转录组。我们的分析表明，在 Bgt 接种前，S 和 R 基因型的小麦原始基因表达状态几乎相同，因为只有 60 个基因的表达差异通过 P = 0.01 截止值。然而，在 Bgt 接种后 12 小时，S 和 R 基因型的小麦分别有 3014 个和 2800 个基因对感染做出响应。涉及到广泛的途径，包括细胞壁加固、类黄酮生物合成和代谢过程。此外，我们首次表明，有义-反义基因对可能是小麦-白粉病相互作用的参与者。此外，对几个候选基因的 qRT-PCR 分析结果与微阵列数据在其表达模式上一致。总之，本研究揭示了小麦近等基因系白粉病感染前后叶片转录组的变化，表明白粉病抗性是一个涉及大量基因调控的高度复杂的系统反应。
