Kolozsváriné Nagy Judit, Schwarczinger Ildikó, Király Lóránt, Bacsó Renáta, Ádám Attila L, Künstler András
Centre for Agricultural Research, Plant Protection Institute, ELKH, 15 Herman Ottó Str., 1022 Budapest, Hungary.
Plants (Basel). 2022 Mar 28;11(7):903. doi: 10.3390/plants11070903.
Barley cultivation is adversely affected by high-temperature stress, which may modulate plant defense responses to pathogens such as barley powdery mildew ( f. sp. Bgh). Earlier research focused mainly on the influence of short-term heat stress (heat shock) of barley on Bgh infection. In this study, our aim was to investigate the effects of both short- and long-term heat stress (35 °C from 30 s to 5 days) on Bgh infection in the barley cultivar Ingrid and its near-isogenic lines containing different powdery mildew resistance genes (, , and ) by analyzing symptom severity and Bgh biomass with RT-qPCR. The expression of selected barley defense genes (, , and ) was also monitored in plants previously exposed to heat stress followed by inoculation with Bgh. We demonstrated that pre-exposure to short- and long-term heat stress negatively affects the resistance of all resistant lines manifested by the appearance of powdery mildew symptoms and increased Bgh biomass. Furthermore, prolonged heat stress (48 and 120 h) enhanced both Bgh symptoms and biomass in susceptible wild-type Ingrid. Heat stress suppressed and delayed early defense gene activation in resistant lines, which is a possible reason why resistant barley became partially susceptible to Bgh.
大麦种植受到高温胁迫的不利影响，高温胁迫可能会调节植物对诸如大麦白粉病（禾本科布氏白粉菌）等病原体的防御反应。早期研究主要聚焦于大麦短期热胁迫（热激）对禾本科布氏白粉菌感染的影响。在本研究中，我们的目的是通过分析症状严重程度以及利用实时定量聚合酶链反应检测禾本科布氏白粉菌生物量，来研究短期和长期热胁迫（35℃，持续30秒至5天）对大麦品种英格丽及其含有不同抗白粉病基因（、和）的近等基因系中禾本科布氏白粉菌感染的影响。还对先前经热胁迫处理后接种禾本科布氏白粉菌的植株中所选大麦防御基因（、、和）的表达进行了监测。我们证明，短期和长期热胁迫预处理会对所有抗性品系的抗性产生负面影响，表现为白粉病症状出现以及禾本科布氏白粉菌生物量增加。此外，延长热胁迫时间（48小时和120小时）会加重感病野生型英格丽中的禾本科布氏白粉菌症状并增加其生物量。热胁迫抑制并延迟了抗性品系中早期防御基因的激活，这可能是抗性大麦对禾本科布氏白粉菌变得部分感病的原因。
