Laboratory of Polymers Engineering for High Technologies (LIPHT), Strasbourg University, ECPM, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France.
Bioresour Technol. 2012 Sep;119:425-8. doi: 10.1016/j.biortech.2012.01.185. Epub 2012 Jun 12.
A purified feruloyl esterase (EC 3.1.1.73) from Aspergillus nidulans produced in Pichia pastoris was used to study the de-esterification of large feruloyl oligosaccharides consisting of 4 to 20 pentose residues and (xylose plus arabinose) and one ferulic acid residue. The feruloyl oligosaccharides were prepared from total oligosaccharidic hydrolysates from wheat bran treated with a purified endoxylanase from Thermobacillus xylanilyticus. The feruloyl esterase showed similar specific activity but an affinity about 3.5-fold higher towards feruloyl oligosaccharides than towards methyl ferulate. Mass spectrometry analysis of the products after long-term enzymatic hydrolyses showed that the esterase was able to hydrolyze the largest feruloyl oligosaccharides and therefore could act alone on feruloyled xylans. Consequently, the feruloyl esterase from A. nidulans could be useful for the enzymatic deconstruction of xylans in plant cell walls.
从里氏木霉中生产的毕赤酵母表达的纯化阿魏酸酯酶（EC 3.1.1.73）用于研究由 4 到 20 个戊糖残基和（木糖加阿拉伯糖）以及一个阿魏酸残基组成的大阿魏酰低聚糖的去酯化。阿魏酰低聚糖是从用热解木聚糖酶（Thermobacillus xylanilyticus）纯化的内切木聚糖酶处理的麦麸总寡糖水解产物中制备的。阿魏酸酯酶对阿魏酰低聚糖的比活性相似，但亲和力约高 3.5 倍。长时间酶解产物的质谱分析表明，该酯酶能够水解最大的阿魏酰低聚糖，因此可以单独作用于阿魏酰木聚糖。因此，来自米曲霉的阿魏酸酯酶可用于植物细胞壁中木聚糖的酶解。
