Department of Plastic and Reconstructive Surgery, Baskent University Faculty of Medicine, Ankara, Turkey.
J Plast Reconstr Aesthet Surg. 2012 Dec;65(12):1712-9. doi: 10.1016/j.bjps.2012.06.011. Epub 2012 Jul 6.
Primary tendon repair aims at increased tensile strength at the time of mobilisation. Tendon repair and regeneration using mesenchymal stem cells have been described in different studies; however, adipose-derived stem cell (ASC) use for tendon regeneration and repair has recently been taken into consideration. In this study, we sought to determine whether ASCs would be beneficial in primary tendon healing.
Both the Achilles tendons of rabbits (n = 6) were incised and consequently repaired. To the left side was applied platelet-rich plasma (PRP) gel and to the right side autologous ASC-mixed PRP. The tensile strength was measured on the 4th week. The samples were taken for immunohistochemical evaluation of collagen type I, transforming growth factor beta (TGF-β) 1, 2, 3, fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF).
The tensile strengths in control and experimental groups were found out to be 29.46 ± 3.66 and 43.06 ± 3.80 kgf. Collagen type I, FGF and VEGF levels were statistically higher, whereas TGF-β1, 2, 3 were lower in the experimental group.
ASCs enhance primary tendon healing; however, the complex interaction and the cascades by which ASCs could increase collagen type I, FGF and VEGF and decrease TGF-β levels should further be investigated.
初级肌腱修复的目的是在活动时增加拉伸强度。在不同的研究中已经描述了使用间充质干细胞进行肌腱修复和再生；然而，最近已经考虑将脂肪来源的干细胞（ASC）用于肌腱再生和修复。在这项研究中，我们试图确定 ASC 是否有益于初级肌腱愈合。
将兔子的跟腱（n=6）双侧切开并修复。左侧应用富含血小板的血浆（PRP）凝胶，右侧应用自体 ASC 混合 PRP。第 4 周测量拉伸强度。采集样本进行 I 型胶原、转化生长因子β（TGF-β）1、2、3、成纤维细胞生长因子（FGF）和血管内皮生长因子（VEGF）的免疫组织化学评估。
对照组和实验组的拉伸强度分别为 29.46±3.66 和 43.06±3.80 kgf。实验组的 I 型胶原、FGF 和 VEGF 水平明显升高，而 TGF-β1、2、3 水平明显降低。
ASC 可增强初级肌腱愈合；然而，ASC 如何增加 I 型胶原、FGF 和 VEGF 并降低 TGF-β 水平的复杂相互作用和级联反应仍需进一步研究。
