Laboratorio de Radiobiología, Centro de Protección e Higiene de las Radiaciones, La Habana, Cuba.
Mutat Res. 2012 Oct 9;748(1-2):60-4. doi: 10.1016/j.mrgentox.2012.06.007. Epub 2012 Jul 5.
The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings.
彗星实验是评估真核细胞 DNA 损伤和修复的最广泛应用的方法之一。彗星可以通过软件进行测量，采用半自动或全自动的方式。在本文中，我们应用 CellProfiler 开源软件对数字化图像中的彗星进行自动分析，报告尾部 DNA 的百分比。CellProfiler 与 CASP 软件的并排比较表明，这两个软件包之间具有良好的一致性。我们的工作表明，使用开源软件对银染彗星进行自动测量是可行的，可显著节省时间。
