Ardeshir Rashid Alijani, Zolgharnein Hossein, Movahedinia Abdolali, Salamat Negin, Zabihi Ebrahim
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, P.O. Box 669, Khorramshahr, Iran.
Department of Marine Biology, Faculty of Marine Sciences, University of Mazandaran, Babolsar, Iran.
Toxicol Rep. 2017 Dec 14;5:113-124. doi: 10.1016/j.toxrep.2017.12.014. eCollection 2018.
The aim of this study was to assess the effects of fipronil insecticide on the Caspian kutum fish at different levels of biological organizations and to find possible relationship between these biomarkers. Different doses of fipronil (65, 130 and 200 mg/kg) were intraperitoneally administered to the fish for 2 weeks. After 7 and 14 days of exposure, alterations in organ-somatic index, tissue and DNA structure, oxidative stress and CYP1A gene expression in gill, liver, brain and kidney were studied. Determination of these parameters in the liver showed that the degree of tissue change (DTC), comet tail, superoxide dismutase (SOD) and relative CYP1A mRNA expression increased mostly in a time dependent manner whereas in the kidney increased mostly in a dose dependent manner. These parameters in the gill increased more in time and dose dependent manner. Apart from the changes in CYP1A expression and oxidative stress, no alterations was observed in the brain. Multiple regression analysis showed that the CYP1A had the most correlation with the organ-somatic index (R = 0.76) and comet tail (R = 0.89) in the liver, and with DTC (R = 0.93) and oxidative stress (R = 0.87) in the kidney. Generally, this study showed that CYP1A gene expression can be considered as one basic factor for fipronil toxicity in this fish. However, other possible factors also should be considered for future research.
本研究的目的是评估氟虫腈杀虫剂在不同生物组织水平上对里海鲟鱼的影响，并找出这些生物标志物之间可能存在的关系。将不同剂量的氟虫腈（65、130和200mg/kg）腹腔注射给鱼，持续2周。在暴露7天和14天后，研究了鳃、肝脏、脑和肾脏的器官-体指数、组织和DNA结构、氧化应激以及CYP1A基因表达的变化。肝脏中这些参数的测定表明，组织变化程度（DTC）、彗星尾、超氧化物歧化酶（SOD）和相对CYP1A mRNA表达大多呈时间依赖性增加，而在肾脏中大多呈剂量依赖性增加。鳃中的这些参数在时间和剂量依赖性方面增加得更多。除了CYP1A表达和氧化应激的变化外，在脑中未观察到任何改变。多元回归分析表明，CYP1A与肝脏中的器官-体指数（R = 0.76）和彗星尾（R = 0.89）以及肾脏中的DTC（R = 0.93）和氧化应激（R = 0.87）相关性最高。总体而言，本研究表明，CYP1A基因表达可被视为该鱼类氟虫腈毒性的一个基本因素。然而，未来的研究还应考虑其他可能的因素。
